Natural Occurrence of Secondary Bacterial Symbionts in Aphids from Tunisia, with a Focus on Genus Hyalopterus
| Autor: | S. Zouari, Monia Kamel Ben Halima, Rosario Gil, Mariana Reyes-Prieto, Amparo Latorre |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Generalitat Valenciana, Consejo Nacional de Ciencia y Tecnología (México), Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Enterobacteriales Hamiltonella defensa Tunisia food.ingredient Arsenophonus Population Zoology Hyalopterus 010603 evolutionary biology 01 natural sciences 03 medical and health sciences food RNA Ribosomal 16S Animals Symbiosis education Phylogeny Ecology Evolution Behavior and Systematics education.field_of_study Ecology biology Aphis spiraecola biology.organism_classification Enterobacteriaceae Parasitoid Secondary symbiont 030104 developmental biology Aphids Insect Science APSE RRNA Operon Buchnera |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1938-2936 0046-225X |
| DOI: | 10.1093/ee/nvy005 |
| Popis: | Aphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identified among many important pest aphid species. To determine interpopulational diversity of S-symbionts, we carried out a survey in a total of 18 populations of six aphid species collected from six localities in Tunisia, by performing a diagnostic polymerase chain reaction analysis of partial 16S-23S rRNA operon sequences. While 61.7% of individuals contained only Buchnera, three S-symbionts were found at different frequencies. Arsenophonus sp. Gherna et al. 1991 (Enterobacteriales: Enterobacteriaceae) was found in all species under study except for Acyrtosiphon pisum (Harris 1776) (Aphidinae: Macrosiphini); Serratia symbiotica Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was present in all analyzed individuals of A. pisum but only sporadically in Aphis spiraecola (Patch 1914) (Aphidinae: Aphidini) and Hyalopterus amygdali (Blanchard 1840) (Aphidinae: Aphidini), while Hamiltonella defensa Moran et al. 2005 (Enterobacteriales: Enterobacteriaceae) was found in all analyzed individuals of one population of Aphis gossypii (Glover 1877) (Aphidinae: Aphidini) and sporadically in two populations of Hyalopterus. The lysogenic bacteriophage APSE-1 (A. pisum secondary endosymbiont, type 1) was detected in the three populations infected with H. defensa. This bacteriophage has been associated with moderate protection against braconid parasitoids in pea aphids. The high prevalence of Arsenophonus sp. in our samples is in accordance with previous studies indicating that, among gammaproteobacteria, this genus is one of the most widespread insect facultative symbionts. This work was supported by grants BFU2015-64322-C2-1-R (co-financed by FEDER funds and Ministerio de Economía y Competitividad, Spain), PrometeoII/2014/065 (Conselleria d’Educació, Generalitat Valenciana, Spain). S. Zouari is a recipient of a fellowship from Tunisian Ministry of Higher Education, and a grant from the Tunisian-Algerian project 1521ICH/RO, 2012 (co-financed by Tunisian and Algerian Ministries of Higher Education). M. Reyes-Prieto is a recipient of a fellowship (number 538243) from Consejo Nacional de Ciencia y Tecnologia, México. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|