The identification and measurement of a new steroid 16 beta-hydroxydehydroepiandrosterone in infant urine
| Autor: | Rodney W. Kelly, P.M. Adhikary, P.J. Sykes, R.A. Harkness, F.L. Mitchell, C.J.W. Brooks, Shackleton Ch |
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| Rok vydání: | 1968 |
| Předmět: |
Pharmacology
Antimony trichloride Chromatography Chromatography Gas Spectrum Analysis Organic Chemistry Clinical Biochemistry Color reaction Infant Newborn Urine Dehydroepiandrosterone Mass spectrometry Biochemistry Paper chromatography chemistry.chemical_compound Endocrinology chemistry Reagent Methods Humans Gas chromatography Solvolysis Chromatography Thin Layer Molecular Biology |
| Zdroj: | Steroids. 12(6) |
| ISSN: | 0039-128X |
| Popis: | Extracts from 1–3 day-old infant urine which had been hydrolysed by Helix pomatia enzyme followed by solvolysis, were subjected to thin-layer chromatography. A band of material giving the 3β-hydroxy- Δ 5 steroid colour reaction with antimony trichloride staining reagent and previously named “U 2 ”, was purified by further thin-layer chromatography and identified as 16β-hydroxydehydroepiandrosterone. Proof of identity was obtained by comparing the chemical and physical characteristics of the chemically synthesized compound, with those of the unknown. Techniques used were: thin-layer and paper chromatography in 5 systems, gas chromatography, mass spectroscopy, infrared spectroscopy and high temperature catalytic reduction. It is excreted in infant urine mainly as a diconjugate. |
| Databáze: | OpenAIRE |
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