Analysis of Mycobacterium africanum in the last 17 years in Aragon identifies a specific location of IS6110 in Lineage 6
| Autor: | Comín, J., Monforte, M.L., Samper, S., Iglesias, M.J., Ibarz, D., Viñuelas, J., Torres, L., Sahagún, J., Lafoz, M.C., Malo, M.C., Otal, I., Aragonese Working Group on Molecular Epidemiology of Tuberculosis (EPIMOLA) |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
DNA Bacterial Tuberculosis Lineage (genetic) Genotyping Techniques Science 030106 microbiology Population Emigrants and Immigrants Article Evolutionary genetics law.invention Genomic analysis 03 medical and health sciences Bacterial Proteins law Polymorphism (computer science) medicine Humans education Genotyping Polymerase chain reaction Retrospective Studies Genetics education.field_of_study Multidisciplinary biology Incidence (epidemiology) Incidence Biological techniques Mycobacterium tuberculosis medicine.disease biology.organism_classification Phylogenetics Molecular Typing Africa Western 030104 developmental biology Spain Sulfurtransferases DNA Transposable Elements Medicine Molecular evolution Genetic techniques Mycobacterium africanum |
| Zdroj: | Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
| Popis: | The purpose of this study was to increase our knowledge about Mycobacterium africanum and report the incidence and characteristics of tuberculosis (TB) due to their lineages in Aragon, Spain, over the period 2003–2019. The study includes all the cases in our region, where all the M. tuberculosis complex isolates are systematically characterised. We detected 31 cases of M. africanum among 2598 cases of TB in the period studied. TB caused by M. africanum is rare (1.19%) in our population, and it affects mainly men of economically productive age coming from West African countries. Among the isolates, Lineage (L) 6 was more frequent than L5. The genotyping of these strains identified five clusters and 13 strains with a unique pattern. The isolates’ characterisation identified a copy of IS6110 within the moaX gene, which turned out to be specific for L6. It will allow the differentiation of this lineage from the rest of MTBC with a simple PCR reaction. It remains to be established whether this polymorphism may limit M. africanum transmission. Furthermore, a mutation in the mutT2 promoter was found as specific for L6 strains, which could be related to the high variability found for L6 compared to L5. |
| Databáze: | OpenAIRE |
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