Characterization of Antithrombotic Activity of Lumbrokinase‐Immobilized Polyurethane Valves in the Total Artificial Heart
| Autor: | Sungmin Jeon, Wongon Kim, Yongdoo Park, Young-Ho Jo, Eunsook Ryu, Jaehee Shim, Jin Hee Kim, Byoung-Goo Min, Hyunjeong Kim, Jaeseung Jeong |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cardiac output
medicine.medical_specialty medicine.medical_treatment Polyurethanes Biomedical Engineering Urology Medicine (miscellaneous) Bioengineering Heart Artificial In Vitro Techniques Biomaterials Coated Materials Biocompatible Fibrinolytic Agents In vivo Endopeptidases Antithrombotic Fibrinolysis medicine Animals Lumbrokinase Thrombus Sheep Chemistry Thrombosis General Medicine Heparin Enzymes Immobilized medicine.disease Heart Valve Prosthesis Fibrinolytic agent Biomedical engineering medicine.drug |
| Zdroj: | Artificial Organs. 23:210-214 |
| ISSN: | 1525-1594 0160-564X |
| Popis: | Thirty ng/mm2 lumbrokinase, a potent fibrinolytic enzyme, was immobilized in a Korean type total artificial heart (KORTAH) valve by photoreaction; polyallylamine was used as a photoreactive linker. Lumbrokinase-immobilized polyurethane valves were then fitted to the total artificial hearts of 3 healthy 50 kg lambs. In the control lamb, the valves were untreated; in one other, only valves on the right were treated; and in the remaining animal, only those on the left. Implants were in place for up to 3 days, and cardiac output was 5 L/min. To facilitate thrombus formation, low doses of heparin were administered. In the control lamb, thrombi was observed only in the inlet parts of the valves. In the other 2 experiments, thrombi formed in untreated control valves but not in lumbrokinase treated valves. The grade of thrombus formation in untreated valves was 1.06+/-1.37 versus 0+/-0 in the treated part by one-sided Student's t-test (p < 0.1). After implantation, fibrinolytic activity was only observed in treated valves by fibrin plate methods. The proteolytic activity of the treated valves was 3 times higher than that of untreated valves using the azocasein method. These data show that lumbrokinase treated polyurethane valves lead to decreased thrombus formation in vivo and that their biocompatibility is therefore greater than that of untreated valves. |
| Databáze: | OpenAIRE |
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