The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
| Autor: | Milan Ešner, Jean-Yvez Tinevez, Veronika Pospíšilová, Radek Fedr, Aleš Hampl, Martin Anger, Iveta Červenková |
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| Přispěvatelé: | Department of Histology and Embryology [Brno] (MED / MUNI), Faculty of Medicine [Brno] (MED / MUNI), Masaryk University [Brno] (MUNI)-Masaryk University [Brno] (MUNI), Central European Institute of Technology [Brno] (CEITEC MU), Brno University of Technology [Brno] (BUT), Institute of Biophysics of the Czech Academy of Sciences (IBP / CAS), Czech Academy of Sciences [Prague] (CAS), Hub d'analyse d'images - Image Analysis Hub (Platform) (IAH), Institut Pasteur [Paris], St. Anne’s University Hospital [Brno], This work was supported by Czech Science Foundation projects 15-11707S and 15-04844S andthe European Regional Development Fund-Project 'National infrastructure for biological and medical imaging' (No.CZ.02.1.01/0.0/0.0/16_013/000 1775)., We thank Drs. Anthony Hyman and Ina Poser (MPICBG, Dresden, Germany) for providing the H2A-GFP BAC construct, Dr. Ronald Naumann (MPI-CBG, Dresden, Germany) for providing the JM8.A mouse ES cells, and Dr. Klara Koudelkova for her excellent technical assistance, Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Genome instability
Multipolar division Cell division Health Toxicology and Mutagenesis [SDV]Life Sciences [q-bio] Cell Biomedical Engineering Spindle assembly checkpoint (SAC) Biology Single-cell tracking General Biochemistry Genetics and Molecular Biology Time-lapse microscopy 03 medical and health sciences 0302 clinical medicine Tissue engineering Artificial Intelligence medicine General Pharmacology Toxicology and Pharmaceutics Mitosis General Immunology and Microbiology General Neuroscience General Medicine Embryonic stem cell 030205 complementary & alternative medicine Cell biology Mitosis length medicine.anatomical_structure 030220 oncology & carcinogenesis General Agricultural and Biological Sciences Embryonic stem (ES) cells Multipolar spindles |
| Zdroj: | Journal of Applied Biomedicine Journal of Applied Biomedicine, University of South Bohemia in České Budějovice, 2019, 17 (4), pp.209-217. ⟨10.32725/jab.2019.018⟩ Journal of Applied Biomedicine, 2019, 17 (4), pp.209-217. ⟨10.32725/jab.2019.018⟩ |
| ISSN: | 1214-021X |
| DOI: | 10.32725/jab.2019.018⟩ |
| Popis: | International audience; Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability. |
| Databáze: | OpenAIRE |
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