Intracellular passage within macrophages affects the trafficking of virulent tubercle bacilli upon reinfection of other macrophages in a serum-dependent manner
| Autor: | K.A. McDonough, M.A. Florczyk, Y. Kress |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pulmonary and Respiratory Medicine
Blood Bactericidal Activity Bacilli Immunology Dose-Response Relationship Immunologic Gene Expression Virulence Vacuole Microbiology Mycobacterium tuberculosis Bacterial Proteins Phagosomes Extracellular Animals Phagosome Chi-Square Distribution biology Macrophages biology.organism_classification Microscopy Electron Electrophoresis Polyacrylamide Gel Bacteria Intracellular |
| Zdroj: | Tubercle and Lung Disease. 80:259-271 |
| ISSN: | 0962-8479 |
| DOI: | 10.1054/tuld.2000.0268 |
| Popis: | Setting : The interaction of tubercle bacilli with macrophages is central to understanding of tuberculosis disease. Objective : The objective was to determine whether prior passage within macrophages affects the behavior of Mycobacterium tuberculosis (Mtb) upon re-entry into other macrophages. Design : Transmission electron microscopy was used to monitor fusion of bacterial phagosomes with late endosomal/lysosomal compartments using thoria as a fluid phase marker. Two-dimensional polyacrylamide gel electrophoresis was used to study bacterial protein expression within macrophages. Results : H37Rv and BCG expressed novel proteins within macrophages. H37Rv also underwent less fusion after intracellular (IC) (24.2±7.7%) than extracellular (XC) (67.4±5.5%) passage when the bacteria entered new macrophages in small clusters. These effects were inhibited by serum, and were not observed with H37Ra or BCG bacteria (78.9±1.6% fused for all conditions). In addition, vacuoles which contained single bacilli were less likely to acquire markers (26.9±2.6%) than those that contained multiple bacilli (77.3±2.8%). Conclusion : These results indicate that phagolysosomal fusion patterns can be modulated by a variety of factors and that virulent Mtb bacteria may express proteins within macrophages that alter their interaction with these host cells. |
| Databáze: | OpenAIRE |
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