Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice
| Autor: | Xiao-le Hu, Honghe Xiao, Yutong Wu, Xiao-feng Wang, Liang Kong, Ji-cong Chen, Jingxian Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Polygala
Neurogenesis 3 6′-disinapoyl sucrose Morris water navigation task Mice Transgenic Biology Pharmacology Hippocampal formation Hippocampus Neuroprotection Other systems of medicine Mice Alzheimer Disease Morris Water Maze Test Neurosphere Amyloid precursor protein Animals Medicine Chinese Traditional Polygala tenuifolia Neural stem cells Molecular Structure Research biology.organism_classification Neural stem cell Mice Inbred C57BL Disease Models Animal nervous system Complementary and alternative medicine biology.protein RZ201-999 |
| Zdroj: | BMC Complementary Medicine and Therapies BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2662-7671 |
| Popis: | Background Alzheimer’s disease (AD) is the most common dementia worldwide, and there is still no satisfactory drug or therapeutic strategy. Polygala tenuifolia is a traditional Chinese medicine with multiple neuroprotective effects. In present study, we investigated the effects of three active constituents [3,6′-disinapoyl sucrose (DISS), onjisaponin B (OB) and tenuifolin (TEN)] of Polygala tenuifolia (PT) on the proliferation and differentiation of neural stem cells (NSCs) to identify the potential active constituent of PT promoting hippocampal neurogenesis. Methods NSCs were isolated from hippocampi of newborn C57BL/6 mice, and transfected with mutant amyloid precursor protein (APP) gene to establish an AD cell model (APP-NSCs). 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were performed, and the proliferation and differentiation of NSCs were assessed by neurosphere formation assay, 5-bromo-2′-deoxyuridine (BrdU) incorporation assay and immunofluorescence (IF) staining analysis. APP/PS1 transgenic mice were administrated with the potential active constituent DISS for 4 weeks. Morris water maze (MWM), Nissl staining assay and IF staining assays were carried out to evaluate the cognitive function, neural damages and hippocampal neurogenesis, respectively. Results DISS exerted the optimal ability to strengthen APP-NSCs proliferation and neuronal differentiation, followed by OB and TEN. Furthermore, DISS treatment for 4 weeks strikingly rescued the cognitive deficits, neuronal injures, and neurogenesis disorder in adult APP/PS1 transgenic mice. Conclusions Our findings demonstrated that DISS is the constituent of PT that triggers the most potent increase of hippocampal neurogenesis in our mouse model of AD. |
| Databáze: | OpenAIRE |
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