Multi-Residue Analysis of Fipronil and Its Metabolites in Eggs by SinChERS-Based UHPLC-MS/MS
| Autor: | Hua Jin, Qi Zhang, Nairui Huo, Keguang Han, Gao Yuanhui, Liu Xiaolin, Jing Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS)
Formic acid rugged Mass spectrometry Quechers Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine effective Sample preparation Food science Fipronil Detection limit fipronil Chromatography Extraction (chemistry) 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Solvent chemistry cheap single-step 030221 ophthalmology & optometry Animal Science and Zoology egg Food Science safe-based method (SinChERS) |
| Zdroj: | Food Science of Animal Resources |
| ISSN: | 2636-0780 2636-0772 |
| Popis: | A method for simultaneous detection of fipronil (F) and its metabolites fipronil desulfinyl (FD), fipronil sulfide (FS), fipronil sulfone (FSO) in chicken eggs was applied and validated. It includes single-step, cheap, effective, rugged, safe-based method (SinChERS) for sample preparation and ultra high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) for chemical analysis. Results suggested that formic acid enhanced the recovery of 4 target residues and 1% supplementation to acetonitrile gained higher recoveries than that of 5%. SinChERS integrated extraction and clean-up steps into one, with shorter time (1.5 h) to operate and higher recoveries (97%–100%) than HLB, Envi-Carb-NH2 and quik-easy-cheap-effective-rugged-safe method (QuEChERS), and it consumed the smallest volume of extracting solvent (10 mL) as QuEChERS. Quantitative analyses using external standard method suggested the linear ranges of 4 target compounds were 1–20 μg/L with R2>0.9947. The limit of detection (S/N>3) and quantification (S/N>10) were 0.3 μg/kg and 1 μg/kg. Recoveries ranged from 89.0% to 104.4%, and the relative standard deviations (n=6) at 1, 10, and 20 μg/kg were lower than 6.03%. Thirty batches of domestic eggs (500 g each) were detected by the established SinChERS-based UHPLC-MS/MS and no target residues were detected in all samples. The method developed in this study is a rapid, sensitive, accurate and economic way for multi-residue analysis of fipronil and its metabolites in eggs. |
| Databáze: | OpenAIRE |
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