RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors
| Autor: | F. R. Bischoff, Dirk Görlich |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
RanBP1
GTP' Transportin Biophysics Receptors Cytoplasmic and Nuclear Importin GTPase Biology Biochemistry Mice GTP-Binding Proteins Structural Biology Genetics Animals Humans RanGAP Molecular Biology Cell Nucleus Nuclear Proteins CAS Biological Transport Cell Biology beta Karyopherins Recombinant Proteins ran GTP-Binding Protein Nucleocytoplasmic Transport Karyopherins Nuclear transport Beta Karyopherins Guanosine Triphosphate |
| Zdroj: | FEBS Letters. 419:249-254 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(97)01467-1 |
| Popis: | Nucleocytoplasmic transport appears mediated by shuttling transport receptors that bind RanGTP as a means to regulate interactions with their cargoes. The receptor-RanGTP complexes are kinetically very stable with nucleotide exchange and GTP hydrolysis being blocked, predicting that a specific disassembly mechanism exists. Here we show in three cases receptor RanGTP x RanBP1 complexes to be the key disassembly intermediates, where RanBP1 stimulates the off-rate at the receptor/RanGTP interface by more than two orders of magnitude. The transiently released RanGTP x RanBP1 complex is then induced by RanGAP to hydrolyse GTP, preventing the receptor to rebind RanGTP. The efficient release of importin beta from RanGTP requires importin alpha, in addition to RanBP1. |
| Databáze: | OpenAIRE |
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