Isolation and Characterization of a Vitellogenin cDNA from Rainbow Trout (Oncorhynchus mykiss) and the Complete Sequence of a Phosvitin Coding Segment
| Autor: | Antonis Goulas, George Taborsky, E. L. Triplett |
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| Rok vydání: | 1996 |
| Předmět: |
endocrine system
DNA Complementary animal structures Molecular Sequence Data Phosvitin Homology (biology) Vitellogenins Vitellogenin Complete sequence Species Specificity Complementary DNA Genetics Animals Amino Acid Sequence Molecular Biology Gene Library chemistry.chemical_classification Genome Base Sequence Sequence Homology Amino Acid biology Sequence Analysis DNA Cell Biology General Medicine biology.organism_classification Molecular biology Amino acid Trout chemistry Oncorhynchus mykiss biology.protein Rainbow trout |
| Zdroj: | DNA and Cell Biology. 15:605-616 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.1996.15.605 |
| Popis: | Phosvitins are extensively phosphorylated serine-rich proteins that are derived from a large hepatic phosphoglycolipoprotein, vitellogenin, and are deposited, after suitable processing, in the eggs of oviparous vertebrates. Despite their widespread occurrence and apparent importance for early embryonic development, very few phosvitins have been sequenced thus far, including no teleost protein. We used a polymerase chain reaction (PCR)-assisted approach to isolate a vitellogenin cDNA clone, complementary to mRNA synthesized in the liver of estrogen-treated rainbow trout. This clone contains a sequence that corresponds to the composition of a phosvitin previously characterized in our laboratory. The amino terminus was identified by amino acid sequencing of the protein. The carboxyl terminus was inferred from homology with other phosvitin-specific sequences from chicken, Xenopus, and lamprey. Trout phosvitin appears to be a small, 53-residue-long protein displaying the long runs of serines that are characteristic of all phosvitins described so far. |
| Databáze: | OpenAIRE |
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