Low abdominal subcutaneous preadipocyte adipogenesis is associated with visceral obesity, visceral adipocyte hypertrophy, and a dysmetabolic state
| Autor: | Julie Lessard, Sofia Laforest, Mathieu Leboeuf, André Tchernof, Line Blackburn, Mélissa Pelletier |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Histology preadipocyte Cellular differentiation Adipose tissue adipogenesis chemistry.chemical_compound visceral adipocyte hypertrophy Internal medicine Adipocyte medicine Oil Red O business.industry visceral obesity Cell Biology Greater omentum medicine.disease medicine.anatomical_structure Endocrinology chemistry Adipogenesis Adipocyte hypertrophy adipogenic differentiation business Dyslipidemia Research Paper |
| Zdroj: | Adipocyte |
| ISSN: | 2162-397X 2162-3945 |
| DOI: | 10.4161/adip.29385 |
| Popis: | Subcutaneous adipose tissue expansion through adipogenesis is increasingly recognized as a major determinant of body fat distribution and obesity-related cardiometabolic alterations. Our objective was to assess whether adipogenic rates of cultured human primary preadipocytes from the visceral and subcutaneous compartments relate to visceral obesity and cardiometabolic alterations. We recruited 35 women undergoing gynecological surgery and assessed body fat distribution by CT as well as fasting plasma lipids and glycemia. Fat samples from the greater omentum and abdominal subcutaneous (SC) compartments were used to assess mature adipocyte cell size and establish primary preadipocyte cultures. Differentiation was induced using adipogenic media and adipogenic rates were assessed using Oil Red O (ORO) absorbance/DNA content ratio and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) activity/DNA of differentiated cells. We found a lower adipogenic capacity of omental (OM) preadipocytes than SC preadipocytes originating from the same women (P < 0.05). Whereas only OM cell size was different among groups of low vs high OM adipogenic rate, SC adipogenic rates were clearly related to increased OM cell size and dyslipidemia when women were separated on median value of either ORO/DNA or G3PDH activity/DNA ratios. When matched for BMI, women with low SC preadipocyte adipogenic rates had a higher visceral adipose tissue area (P < 0.01), omental adipocyte hypertrophy (P < 0.05), higher VLDL-lipid content (P < 0.01) and higher fasting glycemia (P < 0.05) than those with low SC adipogenic rates. In conclusion, low abdominal subcutaneous preadipocyte differentiation capacity in vitro is associated with visceral obesity, visceral adipocyte hypertrophy, and a dysmetabolic state. |
| Databáze: | OpenAIRE |
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