Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming
| Autor: | Robersy Sanchez, Rosemary Schwegel, Kamaldeep S. Virdi, Xiaodong Yang, Tom Maher, Michael J. Axtell, Hardik Kundariya, Sally A. Mackenzie, Isaac J. Dopp |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cellular signalling networks Plant genetics Transgene Science Circadian clock Arabidopsis Inheritance Patterns Gene regulatory network General Physics and Astronomy Biology Histone Deacetylase 6 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Epigenesis Genetic 03 medical and health sciences Quantitative Trait Heritable RNA interference Gene Regulatory Networks DNA (Cytosine-5-)-Methyltransferases Transgenes lcsh:Science Genetic memory (biology) Genetics DNA methylation Multidisciplinary Arabidopsis Proteins Gene Expression Profiling General Chemistry Methylation Plants Genetically Modified Adaptation Physiological MutS DNA Mismatch-Binding Protein Gene Ontology 030104 developmental biology RNA Interference lcsh:Q Reprogramming Genome-Wide Association Study Signal Transduction 010606 plant biology & botany |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-17 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | MSH1 is a plant-specific protein. RNAi suppression of MSH1 results in phenotype variability for developmental and stress response pathways. Segregation of the RNAi transgene produces non-genetic msh1 ‘memory’ with multi-generational inheritance. First-generation memory versus non-memory comparison, and six-generation inheritance studies, identifies gene-associated, heritable methylation repatterning. Genome-wide methylome analysis integrated with RNAseq and network-based enrichment studies identifies altered circadian clock networks, and phytohormone and stress response pathways that intersect with circadian control. A total of 373 differentially methylated loci comprising these networks are sufficient to discriminate memory from nonmemory full sibs. Methylation inhibitor 5-azacytidine diminishes the differences between memory and wild type for growth, gene expression and methylation patterning. The msh1 reprogramming is dependent on functional HISTONE DEACETYLASE 6 and methyltransferase MET1, and transition to memory requires the RNA-directed DNA methylation pathway. This system of phenotypic plasticity may serve as a potent model for defining accelerated plant adaptation during environmental change. Segregation of an MSH1 RNAi transgene produces non-genetic memory that displays transgenerational inheritance in Arabidopsis. Here, the authors compare memory and non-memory full-sib progenies to show the involvement of DNA methylation reprogramming, involving the RdDM pathway, in transition to a heritable memory state. |
| Databáze: | OpenAIRE |
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