Effects of EGF on the mass of inositol 1,4,5-trisphosphate and sn(1,2)-diacylglycerol in freshly isolated rat hepatocytes: Comparison with vasopressin
| Autor: | Paul F. Cerpovicz, Raymond S. Ochs |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Vasopressin Vasopressins Biophysics Neuropeptide Inositol 1 4 5-Trisphosphate In Vitro Techniques Lithium Biology Biochemistry Diglycerides chemistry.chemical_compound Chlorides Epidermal growth factor Internal medicine medicine Animals Inositol Diglyceride Inositol phosphate Egtazic Acid Molecular Biology Protein Kinase C Protein kinase C Diacylglycerol kinase chemistry.chemical_classification Epidermal Growth Factor Cell Biology Rats Enzyme Activation Kinetics Endocrinology Liver chemistry Calcium lipids (amino acids peptides and proteins) Lithium Chloride hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and Biophysical Research Communications. 187:1055-1062 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)91304-9 |
| Popis: | We measured the masses of inositol 1,4,5 trisphosphate (Ins(1,4,5)P3) and diacylglycerol (DAG) in hepatocytes in response to both epidermal growth factor (EGF) and vasopressin. EGF at 25 nM did not alter Ins(1,4,5)P3 content of hepatocytes. However, the combination of 100 nM EGF concentration and incubation with lithium did increase Ins(1,4,5)P3 content. This increase was only one tenth of that elicited by vasopressin in parallel incubations. This finding resolves a controversy concerning the ability of EGF to increase Ins(1,4,5)P3 in hepatocytes, and argues against a role for phosphoinositide hydrolysis in EGF action in hepatocytes. Both EGF and vasopressin caused a rapid (30 s) increase in DAG content. A delayed increase in DAG content, that was maximal after several minutes, was observed only for vasopressin. The rapid increase in DAG content implies an activation of protein kinase C for both EGF and vasopressin. |
| Databáze: | OpenAIRE |
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