The role of actin isoforms in somatic embryogenesis in Norway spruce
| Autor: | Lenka Havelková, Kateřina Schwarzerová, Erica Bellinvia, Petra Boříková, Zdeněk Opatrný, Jindřiška Fišerová, Martin Vágner, Zuzana Vondráková, Lukáš Fischer, Kateřina Eliášová |
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| Jazyk: | angličtina |
| Předmět: |
Gene isoform
Somatic embryogenesis Embryonic Development macromolecular substances Plant Science Biology Genes Plant Tissue Culture Techniques Gene Expression Regulation Plant lcsh:Botany Protein Isoforms Picea Cytoskeleton Actin Phylogeny Binding Sites Embryogenesis Gene Expression Regulation Developmental Amino acid substitution Actin cytoskeleton Bridged Bicyclo Compounds Heterocyclic Molecular biology Actins Cell biology lcsh:QK1-989 Amino Acid Substitution RNA Plant Thiazolidines Sequence Alignment Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 10, Iss 1, p 89 (2010) |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/1471-2229-10-89 |
| Popis: | Background Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs. Results Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching for the cause of the differential actin sensitivity of the two cell types, we analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was greatly enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. Conclusions We show that manipulating actin in specific cell types in somatic embryos using Lat B treatment accelerated and even synchronized the development of somatic embryos and may be of practical use in biotechnology. |
| Databáze: | OpenAIRE |
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