Effect of Fractioning on Antibacterial Activity ofEnantia chloranthaOliver (Annonaceae) Methanol Extract and Mode of Action

Autor: Rosalie Anne Ngono Ngane, Cedric Laurel Cidjeu Pouaha, Jean Paul Assam Assam, François Xavier Etoa, Rebeca Madeleine Ebelle Etame, Igor Voukeng Kenfack, Raphael Tchientcheu, Jules-Roger Kuiate, Raymond Simplice Mouokeu, Alembert Tchinda Tiabou, Frank Stève Monthe Poundeu
Rok vydání: 2018
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018)
ISSN: 1741-4288
1741-427X
Popis: Infectious diseases caused by bacteria constitute the main cause of morbidity and mortality throughout the world and mainly in developing countries. In this work, the influence of fractioning and the mode of action of stem barks methanol extract ofEnantia chloranthawere investigated. The aim was to optimize the antibacterial activity of the methanol extract. The extract was prepared by maceration of barks powder in methanol. Fractioning was done using increasing solvents polarity. Standard phytochemical methods were used for phytochemical screening. Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) of the methanol extract and fractions were determined using broth microdilution method. The studied mode of action of both methanol extract and n-butanol fraction included antibiofilm activity, H+-ATPase-mediated proton pumping assay, salt tolerance, and cells cycle. The methanol extract ofE. chloranthastem barks was found to be active on all the bacteria tested (32 ≤ MIC ≤ 512 μg/mL), its activity being significant (MIC < 100 μg/ml) out of 5 of the 28 clinical isolates used.Salmonella entericaserovar paratyphi A was the most sensitive (32 μg/mL). Compared to the extract and other fractions, the n-butanol fraction was found to be more active (32 ≤ MIC ≤ 256). Significant antibacterial activity of this fraction was observed out of 10 of the 28 bacterial isolates and 3 out of 7 bacterial strains. Lowest MIC values (32 μg/ml) of this fraction were obtained withEscherichia coli(136),Pseudomonas aeruginosa(CIP 76110), andSalmonella entericaserovar typhi 9. The methanol extract ofE. chloranthaand its n-butanol fraction revealed several modes of action including the prolongation of the latency phase of the bacterial growth, the inhibition of the pump with protons H+- ATPases bacterial, the loss of the salt tolerance of theStaphylococcus aureus,and inhibition of the formation of the bacterial biofilm. The present results showed that the n-butanol fraction of the methanol stem barks extract ofE. chloranthapossess the essential antibacterial components and could best be used to fight against bacterial infections as compared to methanol extract.
Databáze: OpenAIRE
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