Overexpression of a Chinese cabbage BrERF11 transcription factor enhances disease resistance to Ralstonia solanacearum in tobacco
| Autor: | Shuilin He, Ailian Qiu, Zhiqin Liu, Youliang Shi, Yuhua Xiao, Jing Lin, Mukun Huang, Yan Lai, Lu Yu, Shaoliang Mou, Deyi Guan, Aihua Qi, Fengfeng Dang, Chengcong Chen, Jinhui Lin |
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| Rok vydání: | 2013 |
| Předmět: |
Hypersensitive response
Physiology Brassica Plant Science Plant disease resistance chemistry.chemical_compound Gene Expression Regulation Plant Arabidopsis Tobacco Genetics Gene Transcription factor Disease Resistance Plant Diseases Plant Proteins Ralstonia solanacearum Methyl jasmonate biology cDNA library fungi food and beverages Plants Genetically Modified biology.organism_classification Cell biology chemistry Transcription Factors |
| Zdroj: | Plant Physiology and Biochemistry. 62:70-78 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2012.10.010 |
| Popis: | Ethylene-responsive factors (ERFs) play diverse roles in plant growth, developmental processes and stress responses. However, the roles and underlying mechanism of ERFs remain poorly understood, especially in non-model plants. In this study, a full length cDNA of ERF gene was isolated from the cDNA library of Chinese cabbage. According to sequence alignment, we found a highly conservative AP2/ERF domain, two nuclear localization signals, and an ERF-associated Amphiphilic Repression (EAR) motif in its C-terminal region. It belonged to VIIIa group ERFs sharing the highest sequence identity with AtERF11 in all of the ERFs in Arabidopsis and designated BrERF11. BrERF11-green fluorescence protein (GFP) transient expressed in onion epidermis cells localized to the nucleus. The transcript levels of BrERF11 were induced by exogenous salicylic acid (SA), methyl jasmonate (MeJA), ethephon (ETH), and hydrogen peroxide (H(2)O(2)). Constitutive expression of BrERF11 enhanced tolerance to Ralstonia solanacearum infection in transgenic tobacco plants, which was coupled with hypersensitive response (HR), burst of H(2)O(2) and upregulation of defense-related genes including HR marker genes, SA-, JA-dependent pathogen-related genes and ET biosynthesis associated genes and downregulation of CAT1, suggesting BrERF11 may participate in pathogen-associated molecular pattern (PAMP)- and effector-triggered immunity (PTI and ETI) mediated by SA-, JA- and ET-dependent signaling mechanisms. |
| Databáze: | OpenAIRE |
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