Endothelialization and Anticoagulation Potential of Surface-Modified PET Intended for Vascular Applications

Autor: Hugo Thienpont, Charles James Kirkpatrick, Heidi Ottevaere, David Schaubroeck, Peter Dubruel, Elena Diana Giol, Ronald E. Unger, Sandra Van Vlierberghe
Přispěvatelé: Applied Physics and Photonics, Brussels Photonics Team, Chemistry
Rok vydání: 2018
Předmět:
Lipopolysaccharides
Polymers and Plastics
Poly(ethylene terephthalate)
Gene Expression
Biocompatible Materials
02 engineering and technology
01 natural sciences
Gelatin
endothelialization
chemistry.chemical_compound
Coating
Polyethylene terephthalate
Materials Chemistry
chemistry.chemical_classification
Polyethylene Terephthalates
Surface modified
hemocompatibility
021001 nanoscience & nanotechnology
Platelet Endothelial Cell Adhesion Molecule-1
0210 nano-technology
E-Selectin
biotechnology
endotoxin content
azide photografting
Azides
food.ingredient
Materials science
Biocompatibility
Cell Survival
Surface Properties
Bioengineering
engineering.material
010402 general chemistry
Biomaterials
food
von Willebrand Factor
Human Umbilical Vein Endothelial Cells
Humans
Tissue Engineering
Biomolecule
Anticoagulants
0104 chemical sciences
Blood Vessel Prosthesis
chemistry
engineering
Surface modification
Blood Vessels
Azide
Biomarkers
Biomedical engineering
Zdroj: Macromolecular bioscience. 18(7)
ISSN: 1616-5195
Popis: In vascular tissue engineering, great attention is paid to the immobilization of biomolecules onto synthetic grafts to increase bio- and hemocompatibility-two critical milestones in the field. The surface modification field of poly(ethylene terephthalate) (PET), a well-known vascular-graft material, is matured and oversaturated. Nevertheless, most developed methods are laborious multistep procedures generally accompanied by coating instability or toxicity issues. Herein, a straightforward surface modification procedure is presented engineered to simultaneously promote surface endothelialization and anticoagulation properties via the covalent immobilization of gelatin through a photoactivated azide derivative. A complete physicochemical characterization and biological study including cytotoxicity and endotoxin testing are performed. In addition, biocompatibility toward small (diameter ≤ 6 mm) and/or large caliber (diameter ≥ 6 mm) vessels is assessed by micro- and macrovascular endothelial cell assays. Superior bio- and hemocompatibility properties are seen for the gelatin-covalently modified PET surfaces compared to the conventional surface-modification procedures based on physisorption.
Databáze: OpenAIRE
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