Direct Radioimmunoassay for Haloperidol in Human Serum
| Autor: | Tetsuya Oida, Akira Kagemoto, Sachiko Oida, Yuzo Utsui, Masashi Nakao, Yoshiaki Terauchi, Yutaka Sekine, Shoko Ishikawa |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Chromatography Gas Immunogen Guinea Pigs Radioimmunoassay Pharmaceutical Science Cross Reactions medicine.disease_cause behavioral disciplines and activities Cross-reactivity Internal medicine medicine Haloperidol Animals Humans Bovine serum albumin Antiserum Vaccines Synthetic biology Chemistry Immune Sera Metabolism Endocrinology biology.protein Hapten medicine.drug |
| Zdroj: | Journal of Pharmaceutical Sciences. 79:432-436 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600790515 |
| Popis: | A direct radioimmunoassay for the accurate determination of haloperidol in human serum has been developed. Based on recent information about the metabolism of haloperidol, a new haloperidol hapten, in which a (3-carboxypropionyl)methylamino group was attached as a bridge in the place of fluorine atom, was synthesized and coupled to bovine serum albumin through the bridge to provide a new immunogen. Guinea pigs were used for the immunization. Since the antisera obtained by the new immunogen still cross reacted greater than 10% with reduced haloperidol, the immunological tolerance to reduced haloperidol was induced by administration of a copolymer of D-glutamic acid and D-lysine linked with reduced haloperidol. This gave an antiserum in guinea pigs which was highly specific for unchanged haloperidol with negligible cross reactivity (less than or equal to 1.0%) to any haloperidol metabolites including the newly found ones. With the newly developed antiserum and [3H]haloperidol, serum haloperidol levels can be determined over the concentration range from 0.3 to 20 ng/mL, using 0.1 mL of human serum, without an extraction procedure. |
| Databáze: | OpenAIRE |
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