Heterogeneous expression of SNARE proteins SNAP-23, SNAP-25, Syntaxin1 and VAMP in human parathyroid tissue
| Autor: | Christina Bark, Anthony J. Gill, Lars Forsberg, Lars-Ove Farnebo, Robert Bränström, Christofer Carl Juhlin, Arthur D. Conigrave, Ming Lu, Catharina Larsson, Anders Höög, Vladana Vukojević, Leigh Delbridge |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Synaptosomal-Associated Protein 25 Blotting Western Parathyroid hormone Fluorescent Antibody Technique Syntaxin 1 Biology Biochemistry Exocytosis Parathyroid Glands R-SNARE Proteins 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Western blot Internal medicine medicine Chief cell Animals Humans Qc-SNARE Proteins Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Aged Aged 80 and over 0303 health sciences medicine.diagnostic_test Life Sciences Munc-18 Parathyroid chief cell Middle Aged Qb-SNARE Proteins medicine.disease Cell biology stomatognathic diseases Parathyroid Neoplasms Parathyroid carcinoma Membrane protein 030220 oncology & carcinogenesis Female SNARE Proteins |
| Zdroj: | Molecular and Cellular Endocrinology Molecular and Cellular Endocrinology, Elsevier, 2008, 287 (1-2), pp.72. ⟨10.1016/j.mce.2008.01.028⟩ |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2008.01.028⟩ |
| Popis: | In regulated exocytosis synaptosomal-associated protein of 25kDa (SNAP-25) is one of the key-players in the formation of SNARE (soluble N-ethylmaleimide-sensitive fusion attachment protein receptor) complex and membrane fusion. SNARE proteins are essentially expressed in neurons, neuroendocrine and endocrine cells. Whether parathyroid cells express these proteins is not known. In this study, we have examined the expression of the SNARE protein SNAP-25 and its cellular homologue SNAP-23, as well as syntaxin1 and VAMP (vesicle-associated membrane protein) in samples of normal parathyroid tissue, chief cell adenoma, and parathyroid carcinoma, using immunohistochemistry and Western blot analysis. SNAP-23 and VAMP were evenly expressed in all studied parathyroid tissues using immunohistochemistry and/or Western blot analysis. SNAP-25 (and Syntaxin1) was not expressed in normal parathyroid tissue, but in approximately 20% of chief cell adenomas, and in approximately 45% of parathyroid carcinoma samples. It is likely that the SNARE proteins SNAP-23 and VAMP play a role in the stimulus-secretion coupling and exocytosis of parathyroid hormone as these proteins were expressed in all of the parathyroid samples we studied. In particular, preferential expression of SNAP-23 rather than SNAP-25 provides an explanation of the high level of PTH secretion that occurs under conditions of low cytoplasmic free Ca(2+) concentration (around 0.1micromol/l). SNAP-25 (and Syntaxin1) appears to be a tumour-specific protein(s) in parathyroid tissues since its expression was restricted to pathological tissues. |
| Databáze: | OpenAIRE |
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