Glycan-Based Flow-Through Device for the Detection of SARS-COV-2
| Autor: | Alexander James Zwetsloot, Panagiotis G. Georgiou, Ashfaq Ahmad, Neil R Anderson, Caroline I. Biggs, Sarojini Pandey, Alexander N. Baker, Dimitris K. Grammatopoulos, Muhammad Hasan, Simone Dedola, Marc Walker, Robert A. Field, Matthew I. Gibson, Anne Straube, Sarah-Jane Richards, Collette S. Guy |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Glycan
Coronavirus disease 2019 (COVID-19) Computer science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Metal Nanoparticles Bioengineering Computational biology 010402 general chemistry Diagnostic tools 01 natural sciences Article rapid diagnostics RS 03 medical and health sciences glycobiology Track disease Polysaccharides Humans QD Instrumentation Pandemics polymers 030304 developmental biology Fluid Flow and Transfer Processes 0303 health sciences biology SARS-CoV-2 Process Chemistry and Technology Spike Protein COVID-19 QP 0104 chemical sciences lateral flow biology.protein glycans flow-through Spike (software development) nanoparticles Gold RA Viral load RC |
| Zdroj: | ACS Sensors |
| ISSN: | 2379-3694 |
| Popis: | The COVID-19 pandemic, and future pandemics, require diagnostic tools to track disease spread and guide the isolation of (a)symptomatic individuals. Lateral-flow diagnostics (LFDs) are rapid and of lower cost than molecular (genetic) tests, with current LFDs using antibodies as their recognition units. Herein, we develop a prototype flow-through device (related, but distinct to LFDs), utilizing N-acetyl neuraminic acid-functionalized, polymer-coated, gold nanoparticles as the detection/capture unit for SARS-COV-2, by targeting the sialic acid-binding site of the spike protein. The prototype device can give rapid results, with higher viral loads being faster than lower viral loads. The prototype’s effectiveness is demonstrated using spike protein, lentiviral models, and a panel of heat-inactivated primary patient nasal swabs. The device was also shown to retain detection capability toward recombinant spike proteins from several variants (mutants) of concern. This study provides the proof of principle that glyco-lateral-flow devices could be developed to be used in the tracking monitoring of infectious agents, to complement, or as alternatives to antibody-based systems.\ud \ud |
| Databáze: | OpenAIRE |
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