Morphology, ultrastructure and immunocytochemistry of Hypnea cervicornis and Hypnea musciformis-(Hypneaceae, Rhodophyta) from the coastal waters of Ceará, Brazil
| Autor: | Emilio C. Miguel, Benildo Sousa Cavada, Silvana Saker-Sampaio, Alexandre Holanda Sampaio, Saulo Pireda, Éder C. Schmidt, Kyria S. Nascimento, Celso Shiniti Nagano, Zenilda L. Bouzon, Fernando Edson Pessoa do Nascimento, Thaiz Batista Azevedo Rangel Miguel, Maura Da Cunha |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
food.ingredient
biology Lectin Hypnea Lectin subcellular localization Red algae biology.organism_classification Pathology and Forensic Medicine Electronic Optical and Magnetic Materials Chloroplast Cell wall food Hypnea musciformis lectin Algae Hypnea cervicornis lectin Algae morphology Nephrology Cytoplasm Ultrastructure Botany biology.protein Instrumentation |
| Zdroj: | Journal of Microscopy and Ultrastructure. (2):104-116 |
| ISSN: | 2213-879X |
| DOI: | 10.1016/j.jmau.2014.03.004 |
| Popis: | Based on their morphological and physiological features, red algae comprise a complex and variable group of multiple genera, including Hypnea. In particular, the genus Hypnea J.V. Lamouroux (Cystocloniaceae, Rhodophyta) consists of approximately 54 species, including Hypnea cervicornis and H. musciformis. Lectins were described for both species; however, the localization of these proteins is still unclear. Therefore, this work aimed to characterize the morphology and ultrastructure of Hypnea cervicornis and H. musciformis, as well as localize their lectins at the subcellular level. Samples were collected at Praia do Pacheco (Fortaleza-CE) and processed for light, scanning and transmission electron microscopy, in addition to immunocytochemistry. The studied species presented cortical cell layers, subcortical cells and medullary cells. Based on ultrastructural analysis, these species presented vacuolated cortical cells, with a dense cytoplasm containing chloroplasts. The cell wall consisted of concentric microfibrils embedded in an amorphous matrix. Immunochemistry analysis showed the expression of lectins in the cytoplasm and cell walls. While the structure of the studied algae was similar to the description of other species of the genera under different conditions, this is the first record of algae lectin localization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|