Rhabdomyosarcoma of Soft Tissues in an Adult Brook Trout (Salvelinus fontinalis)
| Autor: | Giuseppe Sarli, Carlo Bianco, Chiara Brachelente, Rubina Sirri, I. Pulvirenti, Luciana Mandrioli, Paola Beraldo, Marco Galeotti |
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| Přispěvatelé: | Sirri, R, Bianco, C., Beraldo, P., Mandrioli, L., Pulvirenti, I., Brachelente, C., Galeotti, M., Sarli, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Trout Vimentin Soft Tissue Neoplasms Electron Pathology and Forensic Medicine Solid Alveolar Rhabdomyosarcoma Lesion Immunohistochemistry Protein blast Rhabdomyosarcoma Salmonid Animals Biomarkers Tumor Fish Diseases Microscopy Electron Transmission 2734 Veterinary (all) Medicine (all) medicine Transmission Microscopy Tumor General Veterinary biology Histology medicine.disease Primary and secondary antibodies biology.protein Desmin medicine.symptom Biomarkers |
| Popis: | Summary This report describes a spontaneously arising rhabdomyosarcoma of soft tissues in a brook trout (Salvelinus fontinalis). The lesion was examined by means of histology, immunohistochemistry (IHC) and transmission electron microscopy (TEM). The cross-reactivity of the primary antibodies used in the IHC was investigated in silico using the Protein Blast system. Microscopically, the lesion appeared as a ‘small round cell’ undifferentiated sarcoma with rare myotube formation. IHC identified expression of sarcomeric actin and vimentin and these molecules showed the highest protein sequence identity. Lower protein sequence identity coincided with negative immunolabelling for desmin, MyoD1, myogenin and CD3. TEM revealed myofibrils, but without a defined sarcomeric architecture. The diagnosis of solid alveolar rhabdomyosarcoma of soft tissues was achieved on the basis of histological and ultrastructural findings. |
| Databáze: | OpenAIRE |
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