Identification and Characterization of a Newly Isolated Chitinase-Producing Strain Bacillus licheniformis SSCL-10 for Chitin Degradation
Autor: | Nagarajan Duraipandiyan, Noura Al-Dayan, Kannan Marikani, Abirami Sasi, Sugapriya Dhanasekaran, Divya Venugopal |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
DNA
Bacterial 0106 biological sciences Article Subject Physiology Mutant Mutagenesis (molecular biology technique) Chitin 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Crustacea RNA Ribosomal 16S 010608 biotechnology Animals Bacillus licheniformis Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Strain (chemistry) Chemistry Chitinases biology.organism_classification QR1-502 Enzyme Seafood Biochemistry Chitinase biology.protein Artemia Bacteria Research Article |
Zdroj: | Archaea Archaea, Vol 2020 (2020) |
ISSN: | 1472-3646 |
DOI: | 10.1155/2020/8844811 |
Popis: | Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis. A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV. There were 16 mutant strains exhibiting chitinase activity. Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as Bacillus licheniformis (SSCL-10) with the highest specific activity of 3.4 U/mL. The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain. The partially purified chitinase has a molecular weight of 66 kDa. The wild strain (SSCL-10) was identified as Bacillus licheniformis using 16S rRNA sequence analysis. This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry. |
Databáze: | OpenAIRE |
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