Effect of the lactose source on the ultrasound-assisted enzymatic production of galactooligosaccharides and gluconic acid
| Autor: | Laura Ruiz-Aceituno, Mar Villamiel, Antonia Montilla, Fabián Rico-Rodríguez, Juan Carlos Serrato |
|---|---|
| Přispěvatelé: | Colciencias (Colombia), Consejo Superior de Investigaciones Científicas (España), Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2020 |
| Předmět: |
Acoustics and Ultrasonics
medicine.medical_treatment Gluconic acid Oligosaccharides Lactose 02 engineering and technology 010402 general chemistry Gluconates 01 natural sciences Inorganic Chemistry Matrix (chemical analysis) Sonication chemistry.chemical_compound Whey medicine Chemical Engineering (miscellaneous) Environmental Chemistry Monosaccharide Radiology Nuclear Medicine and imaging Glucose oxidase Food science Multienzyme system chemistry.chemical_classification biology Chemistry Galactooligosaccharide Prebiotic Organic Chemistry Galactose Substrate (chemistry) 021001 nanoscience & nanotechnology Enzymes 0104 chemical sciences Prebiotics Process intensification biology.protein Cheese by-products 0210 nano-technology Galactooligosaccharides |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1350-4177 |
| DOI: | 10.1016/j.ultsonch.2019.104945 |
| Popis: | This work is part of the doctoral thesis “Evaluation of a multienzyme system for galactooligosaccharides and gluconic acid production from cheese whey as lactose source”. It is well known that one of the main problems in galactooligosaccharide production (GOS) via tranglycosylation of lactose is the presence of monosaccharides that contribute to increasing the glycaemic index, as is the case of glucose. In this work, as well as studying the effect of ultrasound (US) on glucose oxidase (Gox) activation during gluconic acid (GA) production, we have carried out an investigation into the selective oxidation of glucose to gluconic acid in multienzymatic reactions (β-galactosidase (β-gal) and Gox) assisted by power US using different sources of lactose as substrate (lactose solution, whey permeate, cheese whey). In terms of the influence of matrix on GOS and GA production, lactose solution gave the best results, followed by cheese whey and whey permeate, salt composition being the most influential factor. The highest yields of GOS production with the lowest glucose concentration and highest GA production were obtained with lactose solution in multienzymatic systems in the presence of ultrasound (30% amplitude) when Gox was added after 1 h of treatment with β-gal. This work demonstrates the ability of US to enhance efficiently the obtainment of prebiotic mixtures of low glycaemic index. F. Rico-Rodriguez thanked the Administrative Department of Science and Technology of Colombia – COLCIENCIAS by the grant for Colombian doctoral formation No. 567/2012. L.R-A. thanks the Spanish Research Council (CSIC) and the Spanish Ministry of Economy and Competitiveness for a Juan de la Cierva contract. This work has been funded by MINECO of Spain, Project AGL2014-53445-R. |
| Databáze: | OpenAIRE |
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