Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats
Autor: | A.L.M. Wunderlich, S.C.S.F. Azevedo, L.A. Yamada, C. Bataglini, C. Previate, K.S.S. Campanholi, P.C.S. Pereira, W. Caetano, V. Kaplum, C.V. Nakamura, A.B.S. Nakanishi, J.F. Comar, M.M.D. Pedrosa, V.A.F. Godoi |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Chlorophyll Male Medicine (General) Glycogenolysis Physiology medicine.disease_cause Biochemistry Energy homeostasis 0302 clinical medicine Hyperinsulinemia Glycolysis Biology (General) General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification Photosensitizing Agents Photopigment General Neuroscience Diabetes General Medicine Liver 030220 oncology & carcinogenesis Drug Therapy Combination Research Article medicine.medical_specialty Free Radicals QH301-705.5 Immunology Biophysics Ocean Engineering Diabetes Mellitus Experimental 03 medical and health sciences R5-920 Internal medicine Diabetes mellitus medicine Animals Rats Wistar Reactive oxygen species nutritional and metabolic diseases Cell Biology Metabolism Phototherapy medicine.disease Rats Oxidative Stress 030104 developmental biology Endocrinology chemistry Photochemotherapy Energy Metabolism Oxidative stress |
Zdroj: | Brazilian Journal of Medical and Biological Research v.53 n.1 2020 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Vol 53, Iss 1 (2019) |
Popis: | Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy metabolism and oxidative stress in T1DM Wistar rats was investigated. Evaluation of the acute effects of this pigment was performed by incubation of isolated hepatocytes with chlorophyll-a and the chronic effects were evaluated by oral treatment with chlorophyll-based extract, with post-analysis of the intact liver by in situ perfusion. In both experimental protocols, chlorophyll-a decreased hepatic glucose release and glycogenolysis rate and stimulated the glycolytic pathway in DM/PDT. In addition, there was a reduction in hepatic oxidative stress, noticeable by decreased lipoperoxidation, reactive oxygen species, and carbonylated proteins in livers of chlorophyll-treated T1DM rats. These are indicators of the potential capacity of chlorophyll-a in improving the status of the diabetic liver. |
Databáze: | OpenAIRE |
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