Chlorophyll treatment combined with photostimulation increases glycolysis and decreases oxidative stress in the liver of type 1 diabetic rats

Autor: A.L.M. Wunderlich, S.C.S.F. Azevedo, L.A. Yamada, C. Bataglini, C. Previate, K.S.S. Campanholi, P.C.S. Pereira, W. Caetano, V. Kaplum, C.V. Nakamura, A.B.S. Nakanishi, J.F. Comar, M.M.D. Pedrosa, V.A.F. Godoi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Chlorophyll
Male
Medicine (General)
Glycogenolysis
Physiology
medicine.disease_cause
Biochemistry
Energy homeostasis
0302 clinical medicine
Hyperinsulinemia
Glycolysis
Biology (General)
General Pharmacology
Toxicology and Pharmaceutics
chemistry.chemical_classification
Photosensitizing Agents
Photopigment
General Neuroscience
Diabetes
General Medicine
Liver
030220 oncology & carcinogenesis
Drug Therapy
Combination
Research Article
medicine.medical_specialty
Free Radicals
QH301-705.5
Immunology
Biophysics
Ocean Engineering
Diabetes Mellitus
Experimental
03 medical and health sciences
R5-920
Internal medicine
Diabetes mellitus
medicine
Animals
Rats
Wistar
Reactive oxygen species
nutritional and metabolic diseases
Cell Biology
Metabolism
Phototherapy
medicine.disease
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Photochemotherapy
Energy Metabolism
Oxidative stress
Zdroj: Brazilian Journal of Medical and Biological Research v.53 n.1 2020
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 53, Iss 1 (2019)
Popis: Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy metabolism and oxidative stress in T1DM Wistar rats was investigated. Evaluation of the acute effects of this pigment was performed by incubation of isolated hepatocytes with chlorophyll-a and the chronic effects were evaluated by oral treatment with chlorophyll-based extract, with post-analysis of the intact liver by in situ perfusion. In both experimental protocols, chlorophyll-a decreased hepatic glucose release and glycogenolysis rate and stimulated the glycolytic pathway in DM/PDT. In addition, there was a reduction in hepatic oxidative stress, noticeable by decreased lipoperoxidation, reactive oxygen species, and carbonylated proteins in livers of chlorophyll-treated T1DM rats. These are indicators of the potential capacity of chlorophyll-a in improving the status of the diabetic liver.
Databáze: OpenAIRE
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