The impact of oxidative DNA changes and ATM expression on morphological and functional activities on hepatocytes obtained from mesenchymal stem cells
| Autor: | Shahnaz Esmaeli, Masoud Soleimani, Maryam Adelipour, Mina Allameh, Abdolamir Allameh |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Bioengineering Oxidative phosphorylation Ataxia Telangiectasia Mutated Proteins Biology Applied Microbiology and Biotechnology Oxidative dna damage 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Deoxyguanosine Humans Progenitor cell Pharmacology chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine DNA Molecular biology Cell biology 030104 developmental biology chemistry Gene Expression Regulation 8-Hydroxy-2'-Deoxyguanosine 030220 oncology & carcinogenesis Hepatocytes Functional activity Oxidation-Reduction Biotechnology |
| Zdroj: | Biologicals : journal of the International Association of Biological Standardization. 47 |
| ISSN: | 1095-8320 |
| Popis: | Resistance to oxidative damages in undifferentiated mesenchymal stem cells (MSCs) in comparison with the undifferentiated progenitor cells may differ depending on several factors. This study was carried out to examine the impact of hepatogenic differentiation process of MSCs on oxidative DNA damage markers. Hepatic differentiation of MSCs was carried out using a two-step conventional protocol and the cells were processed for characterization using morphological and biochemical markers. During the course of differentiation cellular levels of reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OH-dG) and expression of ataxia-telangiectasia mutated (ATM) protein were estimated at time intervals (10, 20 and 30 days). The results showed a decrease in cellular ROS (13%, P |
| Databáze: | OpenAIRE |
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