Nanoparticle-based immunosensor with apoferritin templated metallic phosphate label for quantification of phosphorylated acetylcholinesterase
| Autor: | Yuehe Lin, Yunying Xie, Dan Du, Aiqiong Chen, Aidong Zhang |
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| Rok vydání: | 2010 |
| Předmět: |
Biomedical Engineering
Biophysics Nanoparticle Biosensing Techniques Electrochemistry Adduct Phosphates Metal Pesticides Phosphorylation Detection limit Immunoassay Chromatography Chemistry General Medicine Quartz crystal microbalance Environmental Exposure Metals visual_art Electrode Apoferritins visual_art.visual_art_medium Acetylcholinesterase Nanoparticles Biosensor Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 26(9) |
| ISSN: | 1873-4235 |
| Popis: | A new sandwich-like electrochemical immunosensor has been developed for quantification of organophosphorylated acetylcholinesterase (OP-AChE), an exposure biomarker of organophosphate pesticides and nerve agents. Zirconia nanoparticles (ZrO2 NPs) were anchored on a screen printed electrode (SPE) to preferably capture OP-AChE adducts by metal chelation with phospho-moieties, which was selectively recognized by lead phosphate-apoferritin labeled anti-AChE antibody (LPA-anti-AChE). The sandwich-like immunoreactions were performed among ZrO2 NPs, OP-AChE and LPA-anti-AChE to form ZrO2/OP-AChE/LPA-anti-AChE complex and the released lead ions were detected on a disposable SPE. The binding affinity was investigated by both square wave voltammetry (SWV) and quartz crystal microbalance (QCM) measurements. The proposed immunosensor yielded a linear response current over a broad OP-AChE concentrations range from 0.05 nM to 10 nM, with detection limit of 0.02 nM, which has enough sensitivity for monitoring of low-dose exposure to OPs. This method avoids the drawback of unavailability of commercial OP-specific antibody as well as amplifies detection signal by using apoferritin encoded metallic phosphate nanoparticle tags. This nanoparticle-based immunosensor offers a new method for rapid, sensitive, selective and inexpensive quantification of phosphorylated adducts for monitoring of OP pesticides and nerve agents exposures. |
| Databáze: | OpenAIRE |
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