Raised plasma concentrations of alpha-defensins in patients with idiopathic pulmonary fibrosis
| Autor: | Jun-ichi Kadota, Masamitsu Nakazato, H Iiboshi, Masatoshi Tokojima, Takeaki Hiratsuka, Shigeru Matsukura, Hiroshi Mukae, Shigeru Kohno, Jun-ichi Ashitani, Kuniko Abe |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine alpha-Defensins Pathology medicine.medical_specialty Pulmonary Surfactant-Associated Proteins Proteolipids Pulmonary Fibrosis Vital Capacity Radioimmunoassay Pulmonary function testing Pathogenesis Idiopathic pulmonary fibrosis Forced Expiratory Volume Pulmonary fibrosis medicine Humans Glycoproteins Carbon Monoxide medicine.diagnostic_test business.industry fungi Respiratory disease Interstitial lung disease Interleukin hemic and immune systems Pulmonary Surfactants Middle Aged respiratory system Pulmonary Surfactant-Associated Protein D medicine.disease Immunohistochemistry Peptide Fragments respiratory tract diseases Bronchoalveolar lavage Immunology Original Article Female business Bronchoalveolar Lavage Fluid Procollagen |
| Zdroj: | Thorax. 57:623-628 |
| ISSN: | 0040-6376 |
| Popis: | Background: Neutrophils are thought to play an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Human neutrophils contain antimicrobial and cytotoxic peptides in the azurophil granules which belong to a family of mammalian neutrophil peptides named α-defensins. A study was undertaken to investigate the role of α-defensins in the pathogenesis of IPF. Methods: The concentrations of α-defensins (human neutrophil peptides (HNPs) 1, 2, and 3) in plasma and bronchoalveolar lavage (BAL) fluid of 30 patients with IPF and 15 healthy subjects were measured by radioimmunoassay. Results: The concentrations of α-defensins in plasma, but not in BAL fluid, were significantly higher in IPF patients than in controls. BAL fluid concentrations of interleukin (IL)-8 in patients with IPF, which were significantly higher than in controls, correlated with those of α-defensins. An inverse relationship was seen between plasma α-defensin levels and the arterial oxygen tension (PaO2) and pulmonary function (vital capacity (%VC), forced expiratory volume in 1 second (FEV1), and carbon monoxide transfer factor (%TLCO)) in patients with IPF. Plasma levels of α-defensins also correlated with the clinical course in IPF patients with an acute exacerbation. Immunohistochemically, positive staining was observed inside and outside neutrophils in the alveolar septa, especially in dense fibrotic areas. Conclusion: These findings suggest that α-defensins play an important role in the pathogenesis of IPF, and that the plasma α-defensin level may be a useful marker of disease severity and activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|