HIV-1 Tat Protects CD4+ Jurkat T Lymphoblastoid Cells from Apoptosis Mediated by TNF-Related Apoptosis-Inducing Ligand
| Autor: | Maria Carla Re, Alessandra Cappellini, Cristina Ponti, Claudia Maldini, Giorgio Zauli, Michele La Placa, Claudio Celeghini, Davide Gibellini |
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| Přispěvatelé: | Gibellini, D, Re, Mc, Ponti, Cristina, Maldini, C, Celeghini, Claudio, Cappellini, A, LA PLACA, M, Zauli, G. |
| Rok vydání: | 2001 |
| Předmět: |
HIV-1
Tat TRAIL apoptosis medicine.medical_treatment Immunology TNF TRAIL Biology Transfection Jurkat cells Receptors Tumor Necrosis Factor NO TNF-Related Apoptosis-Inducing Ligand Jurkat Cells Plasmid In Situ Nick-End Labeling Extracellular medicine Humans Membrane Glycoproteins HIV-1 apoptosis TNF Tumor Necrosis Factor-alpha Lymphoblast apoptosis Molecular biology Receptors TNF-Related Apoptosis-Inducing Ligand Cytokine Apoptosis Gene Products tat HIV-1 Tat tat Gene Products Human Immunodeficiency Virus Tumor necrosis factor alpha Apoptosis Regulatory Proteins |
| Zdroj: | Cellular Immunology. 207:89-99 |
| ISSN: | 0008-8749 |
| DOI: | 10.1006/cimm.2000.1746 |
| Popis: | We have here investigated the effect of TNF-related apoptosis-inducing ligand (TRAIL), a new member of the TNF cytokine superfamily, on the survival of Jurkat lymphoblastoid cell lines stably transfected with plasmids expressing the wild-type or mutated (Cys22) human immunodeficiency virus type 1 (HIV-1) tat gene. Jurkat cells transfected with wild-type tat were resistant to TRAIL-mediated apoptosis, while Jurkat cells mock-transfected with the control plasmid or with a mutated nonfunctional tat cDNA were highly susceptible to TRAIL-mediated apoptosis. Also, pretreatment with low concentrations (10–100 ng/ml) of extracellular synthetic Tat protein partially protected Jurkat cells from TRAIL-mediated apoptosis. Taken together, these results demonstrated that endogenously expressed tat and, to a lesser extent, extracellular Tat block TRAIL-mediated apoptosis. Since it has been shown that primary lymphoid T cells purified from HIV-1-infected individuals are more susceptible than those purified from normal individuals to TRAIL-mediated apoptosis, our findings underscore a potentially important role of Tat in protecting HIV-1-infected cells from TRAIL-mediated apoptosis. |
| Databáze: | OpenAIRE |
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