Ultrastructural variations of rat myocardium due to Walterinnesia aegyptia snake envenomation
| Autor: | Saad S. Al-Saleh, Fahad J. Al-Shammary, Sameh E. El-Shewemi |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Pathology medicine.medical_specialty Contraction (grammar) medicine.medical_treatment Intraperitoneal injection Venom chemistry.chemical_compound medicine Animals Myocyte Instrumentation Elapid Venoms Glycogen biology Myocardium Heart Anatomy biology.organism_classification Rats Microscopy Electron chemistry Snake venom Walterinnesia aegyptia Ultrastructure Muscle Contraction |
| Zdroj: | Journal of Electron Microscopy. 47:637-643 |
| ISSN: | 1477-9986 0022-0744 |
| DOI: | 10.1093/oxfordjournals.jmicro.a023637 |
| Popis: | This study was undertaken to investigate the toxic effect of Walterinnesia aegyptia venom on the ultrastructure of rat myocardium. Male albino rats were prepared for intraperitoneal injection of saline (control group) and saline solution of W.aegyptia venom (study group) at a dose of 0.04 mg animal-1. Biopsies from the left ventricle were prepared for electron microscopy after 1 h (D1 group), 2 h (D2 group), 18 h (D3 group) and 24 h (D4 group). Myocardial cells were in a state of partial to complete contraction. The D1 group showed some mitochondrial vacuoles; D2 group demonstrated more vacuolation and alterations in the form of disorganized cristae. Similar findings were depicted in D3 group. The D4 group demonstrated, in addition, dissolution of mitochondrial cristae. Myofilaments in D3 group experienced coalescence into ill-defined amorphous masses (foci of myolysis). These masses were characterized by the presence of multiple, parallel, Z-like dark bands with disorganization of the filamentous arrangement. In the D4 group, more myolytic foci were observed. This reaction was not limited to one myocyte but extended to the neighbouring ones. Mitochondrial vacuoles were mostly associated with electron dense deposits. Glycogen particles tended to decrease as the experiment proceeded from D1 to D4. These ultrastructural changes were time dependent. They would suggest a cardiotoxic action of W.aegyptia snake venom. |
| Databáze: | OpenAIRE |
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