Adhesion ofStreptococcus sanguinis to glass surfaces measured by isothermal microcalorimetry (IMC)
| Autor: | Irmgard Hauser-Gerspach, Patricia Scandiucci de Freitas, Jürg Meyer, A. U. Dan Daniels |
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| Rok vydání: | 2008 |
| Předmět: |
Isothermal microcalorimetry
Carbonyl Cyanide m-Chlorophenyl Hydrazone Saliva Materials science Surface Properties Dental Plaque Biomedical Engineering Calorimetry Dental plaque Bacterial Adhesion Microbiology Biomaterials In vivo Human tooth medicine Humans biology Uncoupling Agents Biofilm Adhesion medicine.disease biology.organism_classification Streptococcus sanguinis medicine.anatomical_structure Biofilms Biophysics Glass Streptococcus sanguis |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. :42-49 |
| ISSN: | 1552-4981 1552-4973 |
| Popis: | Bacterial adhesion is the first step in the development of the oral biofilm, called dental plaque. Plaque is the cause of caries, periodontal diseases, and periimplantitis. Investigations of dental plaque, including bacterial adhesion, employ various in vivo and invitro models using microscopic methods. Microcalorimetry offers another direct approach. The model organism Streptococcus sanguinis is one of the first colonizers adhering to the saliva-coated human tooth surfaces or dental materials within minutes after tooth cleaning. TAM III™ thermostats, equipped with microcalorimeters, were used for isothermal microcalorimetric (IMC) measurements of heat production as a function of time, expressed by power–time (p–t) curves. Continuous measurements of heat production of growing S. sanguinis cells showed their overall metabolic activity and were highly reproducible. For the adhesion experiments the bacteria were allowed to adhere to different amounts of glass beads. Growing S. sanguinis cells produced a characteristic p–t curve with a maximum of 500 μW at 4.5 h when reaching 109 cells ml−1. The same number of stationary S. sanguinis cells, suspended in PBS produced only ∼30 μW at 0.5 h due to adhesion. But the amount of heat increased with available glass surface area, indicating that a portion of the heat of adhesion was measured. Similar results were obtained with stationary S. sanguinis cells suspended in human saliva. This study shows that microcalorimetric evaluation of initial bacterial adhesion is indeed possible and may become a rapid, reproducible screening method to study adhesion of different bacteria to different dental materials or to modified surfaces. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008 |
| Databáze: | OpenAIRE |
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