Discrete separation of HDL2 from HDL3 of human serum by means of polyacrylamide gel
| Autor: | Olga Terebus-Kekish, C. Chester Stock, Marion Barclay |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Adult
Male Tris Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Analytical chemistry General Medicine Middle Aged Biochemistry chemistry.chemical_compound Humans Electrophoresis Polyacrylamide Gel Female lipids (amino acids peptides and proteins) Lipoproteins HDL Polyacrylamide gel electrophoresis Densitometry |
| Zdroj: | Clinica Chimica Acta. 88:9-13 |
| ISSN: | 0009-8981 |
| Popis: | A relatively short, simple procedure is presented to separate serum high-density lipoproteins discretely into the two main classes, those with densities between 1.063 and 1.125 g/ml (HDL2) and those with densities between 1.125 and 1.210 g/ml (HDL3). A 3.5% polyacrylamide gel in 10 cm glass tubes and the use of Tris/glycine buffer, pH 8.4, will accomplish this separation. The components can be identified in several different ways, examples of which are given. This procedure will give rapid and reliable estimations of both HDL2 and HDL3, and can be used to relate their levels and proportional amounts to incidence or risk of atherosclerosis, coronary-artery disease and possibly cancer. |
| Databáze: | OpenAIRE |
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