Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage
| Autor: | Bouchra El Amiri, Xavier Druart, Larbi Allai, Anass Benmoula, Mehmet Öztürk, Boubker Nasser |
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| Přispěvatelé: | Centre Régional de la Recherche Agronomique de Settat, Institut National de la Recherche Agronomique du Maroc (INRA), Université Hassan 1er [Settat], Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Muğla Sıtkı Koçman University, Partenaires INRAE, Campus France through two PRAD ( programme de Recherche Agronomique pour le Développement) projects, CNRST - Maroc (Centre National de la Recherche Scientifique et Technique), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
Male
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] food.ingredient Time Factors Semen DNA Fragmentation Biology Sperm Preservation law.invention antioxidant effects Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology food Food Animals law Skimmed milk Animals Food science Sperm motility Opuntia ficus indica phenolic compound 030219 obstetrics & reproductive medicine Sheep Plant Extracts Extender 0402 animal and dairy science Opuntia 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sperm Spermatozoa Semen Analysis Biochemistry chemistry acetone extracts DNA fragmentation Animal Science and Zoology Lipid Peroxidation ram semen Semen Preservation |
| Zdroj: | Animal Reproduction Science Animal Reproduction Science, Elsevier Masson, 2016, 175, pp.1-9. ⟨10.1016/j.anireprosci.2016.09.013⟩ |
| ISSN: | 1873-2232 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2016.09.013⟩ |
| Popis: | International audience; The present study aimed to assess the phenolic composition of the acetone extract from Opuntia ficus indica cladodes (ACTEX) and its effects on ram semen variables, lipid peroxidation and DNA fragmentation during liquid storage at 5 °C for up to 72 h in skim milk and Tris egg yolk extenders. Semen samples from five rams were pooled extended with Tris-egg yolk (TEY) or skim milk (SM) extenders containing ACTEX (0%, 1%, 2%, 4% and 8%) at a final concentration of 0.8 × 109 sperm/ml and stored for up to 72 h at 5 °C. The sperm variables were evaluated at different time periods (8, 24, 48 and 72 h). Sperm total motility and viability were superior in TEY than in SM whereas the progressive motility, membrane integrity, abnormality and spontaneous lipid peroxidation were greater in SM compared to TEY (P< 0.05). The results also indicated that the inclusion of 1% ACTEX in the SM or TEY extender increased the sperm motility, viability, membrane integrity, and decreased the abnormality, lipids peroxidation up to 72 h in storage compared to control group. Similarly, even at 72 hours of storage, 1% ACTEX can efficiently decrease the negative effects of liquid storage on sperm DNA fragmentation (P |
| Databáze: | OpenAIRE |
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