Modified natural porcine surfactant modulates tobacco smoke-induced stress response in human monocytes
| Autor: | H Walti, Maria Bachelet, Barbara S. Polla, Dominique François, Françoise Pinot |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Swine
medicine.disease_cause Monocytes General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Pulmonary surfactant Superoxides Smoke Heat shock protein Tobacco medicine Animals Humans HSP70 Heat-Shock Proteins Pseudopodia General Pharmacology Toxicology and Pharmaceutics Phospholipids Cell Size Biological Products Chemistry Superoxide Monocyte Cell Membrane Pulmonary Surfactants General Medicine Flow Cytometry Hsp70 Cell biology Microscopy Electron Oxidative Stress Plants Toxic medicine.anatomical_structure Gene Expression Regulation Biochemistry Vacuolization Vacuoles Toxicity Oxidative stress |
| Zdroj: | Life Sciences. 64:125-134 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(98)00542-6 |
| Popis: | Tobacco smoke (TS) is a potent source of oxidants and oxidative stress is an important mechanism by which TS exerts its toxicity in the lung. We have shown that TS induces heat shock (HS)/stress protein (HSP) synthesis in human monocytes. Pulmonary surfactant (PS) whose major physiological function is to confer mechanical stability to alveoli, also modulates oxidative metabolism and other pro-inflammatory functions of monocytes-macrophages. In order to determine whether PS alters the stress response induced by TS, we incubated human peripheral blood monocytes overnight with modified natural porcine surfactant (Curosurf) (1 mg/ml) before exposure to TS. Curosurf decreased TS-induced, but not HS-induced, expression of the major cytosolic, inducible 72 kD HSP (Hsp70). Furthermore, TS-generated superoxide anions production was significantly decreased by Curosurf in an acellular system, suggesting a direct scavenging effect of PS. We also examined the effects of TS and PS on monocytes ultrastructure. Monocytes incubated with Curosurf presented smoother cell membranes than control monocytes, while TS-induced monocyte vacuolization was, at least in part, prevented by Curosurf. Taken together, our data suggest that PS plays a protective role against oxygen radical-mediated, TS-induced cellular stress responses. |
| Databáze: | OpenAIRE |
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