Bronchoalveolar lavage is an ideal tool in evaluation of local immune response of pigs vaccinated with Pasteurella multocida bacterin vaccine
| Autor: | Nagendra Nath Barman, Shiney George, Anjan Jyoti Nath, Bhupen Sarma |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
pig
Veterinary medicine animal diseases Population SF1-1100 Microbiology Immune system isotype specific enzyme linked immunosorbent assay pulmonary immune response SF600-1100 medicine bronchoalveolar lavage education education.field_of_study General Veterinary medicine.diagnostic_test biology business.industry Antibody titer respiratory system P. multocida Animal culture Vaccination Titer Bronchoalveolar lavage Immunization Immunology biology.protein Antibody business cell types Research Article |
| Zdroj: | Veterinary World Veterinary World, Vol 8, Iss 4, Pp 438-442 (2015) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | AIM The aim was to study the bronchoalveolar lavage (BAL) technique in evaluating the local immune response of pig immunized with Pasteurella multocida bacterin vaccine. MATERIALS AND METHODS Weaned piglets were immunized with formalin-inactivated P52 strain of P. multocida bacterin and evaluated for pulmonary immune response in BAL fluid. BAL was performed before vaccination and at different post vaccination days. The BAL fluid was assayed using enzyme-linked immunosorbent assay to study the development of P. multocida specific antibody isotypes and also evaluated for different cell populations using standard protocol. RESULTS The average recovery percentage of BAL fluid varies from 58.33 to 61.33 in vaccinated and control group of piglets. The BAL fluid of vaccinated pigs showed increase in antibody titer up to 60(th) days post vaccination (8.98±0.33), IgG being the predominant isotype reached maximum titer of 6.12±0.20 on 45(th) days post vaccination, followed by IgM and a meager concentration of IgA could be detected. An increased concentration of the lymphocyte population and induction of plasma cells was detected in the BAL fluid of vaccinated pigs. CONCLUSION Though intranasal vaccination with P. multocida plain bacterin vaccine could not provoke a strong immune response, but is promising as lymphocyte population was increased and plasma cells were detected. BAL can be performed repeatedly up to 3/4 months of age in pigs to study pulmonary immune response without affecting their health. |
| Databáze: | OpenAIRE |
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