A Novel Lipothrixvirus, SIFV, of the Extremely Thermophilic Crenarchaeon Sulfolobus
| Autor: | Jakob K Kristjanson, Ingelore Holz, Ulrike Ziese, Karen E. Nelson, Hans Peter Arnold, T. Utterback, Jan F Weidmann, Marie Crosby, Hans-Peter Klenk, Wolfram Zillig, Claire M. Fraser |
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| Rok vydání: | 2000 |
| Předmět: |
archaea
genome sequence viruses Molecular Sequence Data Genome Viral virion structure lipid envelope Genome Sulfolobus Open Reading Frames Viral Proteins chemistry.chemical_compound Viral envelope Virology Amino Acid Sequence ORFS Genetics Whole genome sequencing Base Sequence Sequence Homology Amino Acid biology Crenarchaeota Helicase Sequence Analysis DNA biology.organism_classification Open reading frame chemistry DNA Viral Viruses biology.protein Electrophoresis Polyacrylamide Gel Sequence Alignment DNA |
| Zdroj: | Virology. 267(2):252-266 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1999.0105 |
| Popis: | We describe a novel lipothrixvirus, SIFV, of the crenarchaeotal archaeon Sulfolobus islandicus. SIFV ( S . i slandicus f ilamentous v irus) has a linear virion with a linear double-stranded DNA genome. These two features coincide in several crenarchaeotal but not in any other viruses. The SIFV core is formed by a zipper-like array of DNA-associated protein subunits and is covered by a lipid envelope containing host lipids. We sequenced ∼96% of the virus genome excepting the DNA termini, which were modified in an unusual, yet uncharacterized, manner. Both, the 5′ and the 3′ DNA termini were insensitive to enzymatic degradation and labelling. Two open reading frames (ORFs) of the SIFV genome are likely to encode helicases and resemble uncharacterized ORFs from other archaea in sequence. Three ORFs showed sequence similarity with each other and each contained a glycosyl transferase motif. Another ORF of the SIFV genome showed significant sequence similarity to the ORF a291 from the well characterized, spindle-shaped Sulfolobus virus SSV1. Due to its structure, SIFV is classified as a lipothrixvirus. |
| Databáze: | OpenAIRE |
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