Effect of hyperbaric oxygenation on mitochondrial function of neuronal cells in the cortex of neonatal rats after hypoxic-ischemic brain damage

Autor: N. Hu, Mingyan Hei, L. Yang, J.J. Dai, X.Y. Xiang
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Medicine (General)
Time Factors
Physiology
Mitochondrion
Biochemistry
Hypoxia ischemia
Rats
Sprague-Dawley
Random Allocation
0302 clinical medicine
Neonatal
General Pharmacology
Toxicology and Pharmaceutics
Biology (General)
lcsh:QH301-705.5
Hypoxia-ischemia
Cerebral Cortex
Neurons
lcsh:R5-920
Hyperbaric Oxygenation
General Neuroscience
Brain
General Medicine
Mitochondria
medicine.anatomical_structure
Cerebral cortex
Anesthesia
Hypoxia-Ischemia
Brain
Female
medicine.symptom
lcsh:Medicine (General)
QH301-705.5
Hyperbaric oxygenation
Immunology
Biophysics
Ocean Engineering
Brain damage
Rats sprague dawley
03 medical and health sciences
R5-920
medicine
Animals
Clinical Investigation
Normal control
Hypoxic ischemic
business.industry
Cell Biology
Rats
Disease Models
Animal
030104 developmental biology
lcsh:Biology (General)
Animals
Newborn
Rat
Mitochondrial function
business
030217 neurology & neurosurgery
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 49, Issue: 5, Article number: e5187, Published: 26 APR 2016
Brazilian Journal of Medical and Biological Research
Brazilian Journal of Medical and Biological Research v.49 n.5 2016
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 49, Iss 5 (2016)
Popis: The timing and mechanisms of protection by hyperbaric oxygenation (HBO) in hypoxic-ischemic brain damage (HIBD) have only been partially elucidated. We monitored the effect of HBO on the mitochondrial function of neuronal cells in the cerebral cortex of neonatal rats after HIBD. Neonatal Sprague-Dawley rats (total of 360 of both genders) were randomly divided into normal control, HIBD, and HIBD+HBO groups. The HBO treatment began immediately after hypoxia-ischemia (HI) and continued once a day for 7 consecutive days. Animals were euthanized 0, 2, 4, 6, and 12 h post-HI to monitor the changes in mitochondrial membrane potential (ΔΨm) occurring soon after a single dose of HBO treatment, as well as 2, 3, 4, 5, 6, and 7 days post-HI to study ΔΨm changes after a series of HBO treatments. Fluctuations in ΔΨm were observed in the ipsilateral cortex in both HIBD and HIBD+HBO groups. Within 2 to 12 h after HI insult, the ΔΨm of the HIBD and HIBD+HBO groups recovered to some extent. A secondary drop in ΔΨm was observed in both groups during the 1-4 days post-HI period, but was more severe in the HIBD+HBO group. There was a secondary recovery of ΔΨm observed in the HIBD+HBO group, but not in the HIBD group, during the 5-7 days period after HI insult. HBO therapy may not lead to improvement of neural cell mitochondrial function in the cerebral cortex in the early stage post-HI, but may improve it in the sub-acute stage post-HI.
Databáze: OpenAIRE
Externí odkaz: