Detection and quantification of honey adulteration via direct incorporation of sugar syrups or bee-feeding: preliminary study using high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and chemometrics
| Autor: | Marie-Claude Clément, Júlio Sancho Linhares Teixeira Militão, Daniel Cabrol-Bass, Christophe B.Y. Cordella, Patrick Drajnudel |
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| Přispěvatelé: | Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Université de Nice Sophia-Antipolis (UNSA), Universidade Federal de Rondônia [Brésil] (UNIR) |
| Rok vydání: | 2005 |
| Předmět: |
Apiary
Organoleptic honey 01 natural sciences Biochemistry Analytical Chemistry Chemometrics 0404 agricultural biotechnology sugar syrups [SDV.IDA]Life Sciences [q-bio]/Food engineering Partial least squares regression Linear regression [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Environmental Chemistry Sugar Spectroscopy Chromatography Chemistry 010401 analytical chemistry anionic chromatography 04 agricultural and veterinary sciences chemometrics Linear discriminant analysis adulteration 040401 food science Amperometry 0104 chemical sciences bee-feeding |
| Zdroj: | Analytica Chimica Acta Analytica Chimica Acta, Elsevier Masson, 2005, 531 (2), pp.239-248. ⟨10.1016/j.aca.2004.10.018⟩ |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2004.10.018 |
| Popis: | Honey adulteration is a complex problem which currently has a significant economic impact and undeniable nutritional and organoleptic consequences. This paper describes the development of an effective anionic chromatographic method (HPAEC-PAD) for honey analysis and adulteration detection. The method relies on the use of chemometric methods to process chromatograms in order to achieve a better discrimination between authentic and adulterated honeys by linear discriminant analysis and to quantify adulteration levels by partial least squares analysis. This approach was investigated using honey samples adulterated from 10 to 40% with various industrial bee-feeding sugar syrups. Good results were obtained in the characterization of authentic and adulterated samples (96.5% of good classification) using linear discriminant analysis followed by a canonical analysis. The application of the partial least squares modeling method provided a corresponding linear regression model allowing the percentage of adulteration of new samples to be estimated directly from sample chromatograms. Additionally, a bee-feeding experiment on a small apiary was conducted in order to evaluate the effect of supplying hives with bee-feeding syrups. This practice is specific to the apicultural area. It has been demonstrated that bee-feeding can modify the sugar composition of the produced honey if it is conducted without safeguards. |
| Databáze: | OpenAIRE |
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