Distinct Characteristics of Resistance to Borrelia burgdorferi-Induced Arthritis in C57BL/6N Mice
| Autor: | Janis J. Weis, Kathleen Petri Seiler, John H. Weis, Cory Teuscher, Ernst J. Eichwald, Ying Ma |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Ratón Immunology Colony Count Microbial Arthritis Spirochaetaceae macromolecular substances Tarsus Animal Microbiology Polymerase Chain Reaction Host-Parasite Interactions Mice Lyme disease Borrelia burgdorferi Group Immunity medicine Animals Borrelia burgdorferi Arthritis Infectious Lyme Disease Mice Inbred BALB C Mice Inbred C3H biology Infectious dose Bacterial Infections biology.organism_classification medicine.disease Antibodies Bacterial Immunity Innate Mice Inbred C57BL Infectious Diseases Immunoglobulin M Immunoglobulin G biology.protein Parasitology |
| Popis: | Studies of mice infected with Borrelia burgdorferi have indicated that the severity of arthritis is influenced by the genetic composition of the host: the C3H mouse develops severe arthritis while BALB/c and C57BL/6 mice develop mild arthritis. In this study, the effects of increasing infectious dose on the severity of arthritis were determined in these three mouse strains. C3H/He mice developed severe arthritis at all infectious doses, with 100% infection requiring 200 spirochetes. In BALB/cAnN mice, arthritis severity was dependent on infectious dose; symptoms were mild with infection by 200 B. burgdorferi and progressively more severe with increasing infectious dose. Infection of BALB/cAnN mice with 2 × 10 4 B. burgdorferi resulted in arthritis with severity identical to that in C3H/He mice. Spirochete levels in rear ankle joints of C3H/HeJ and C3H/HeN mice were relatively high, as detected by PCR, and did not increase with infectious dose. Spirochete levels in joints from BALB/cAnN mice increased with increasing infectious dose to levels found in severely arthritic C3H/He mice. Thus, resistance to severe arthritis in BALB/cAnN mice was conditional: it could be overcome by high infectious dose and the arthritis became severe when high levels of B. burgdorferi were present in joints. A unique response to increasing infectious dose was seen in C57BL/6N mice, which displayed mild to moderate arthritis at all doses of B. burgdorferi tested, up to 2 × 10 5 . At all infectious doses, the levels of spirochetes in ankle joints of C57BL/6N mice were high, equivalent to those found in the severely arthritic C3H/He mice. The arthritis observed in infected (C57BL/6N × C3H/HeN)F 1 mice was of severity intermediate between those of the two parental strains. The finding that resistance to severe arthritis in C57BL/6N mice could not be overcome by high infectious doses and was independent of spirochete levels in joints suggested that it was mediated by a distinct mechanism from that operating in BALB/cAnN mice. |
| Databáze: | OpenAIRE |
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