The First Green Lineage cdc25 Dual-Specificity Phosphatase
| Autor: | Evelyne Derelle, B. Khadaroo, Conchita Ferraz, André Picard, S. Eychenié, Richard G. Cooke, Steven Robbens, G. Peaucellier, Michel Delseny, Hervé Moreau, Y. Van de Peer, Jacques G. Demaille |
|---|---|
| Přispěvatelé: | Biologie intégrative des organismes marins (BIOM), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
Cdc25 [SDV]Life Sciences [q-bio] Phosphatase 01 natural sciences Ostreococcus tauri Dephosphorylation 03 medical and health sciences Cyclin-dependent kinase Dual-specificity phosphatase Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences biology Cell Biology biology.organism_classification 3. Good health Cell biology enzymes and coenzymes (carbohydrates) Schizosaccharomyces pombe biology.protein Phosphorylation biological phenomena cell phenomena and immunity 010606 plant biology & botany Developmental Biology |
| Zdroj: | Cell Cycle Cell Cycle, Taylor & Francis, 2004, 3 (4), pp.511-516. ⟨10.4161/cc.3.4.815⟩ ResearcherID |
| ISSN: | 1538-4101 1551-4005 |
| DOI: | 10.4161/cc.3.4.815⟩ |
| Popis: | The Cdc25 protein phosphatase is a key enzyme involved in the regulation of the G(2)/M transition in metazoans and yeast. However, no Cdc25 ortholog has so far been identified in plants, although functional studies have shown that an activating dephosphorylation of the CDK-cyclin complex regulates the G(2)/M transition. In this paper, the first green lineage Cdc25 ortholog is described in the unicellular alga Ostreococcus tauri. It encodes a protein which is able to rescue the yeast S. pombe cdc25-22 conditional mutant. Furthermore, microinjection of GST-tagged O. tauri Cdc25 specifically activates prophase-arrested starfish oocytes. In vitro histone H1 kinase assays and anti-phosphotyrosine Western Blotting confirmed the in vivo activating dephosphorylation of starfish CDK1-cyclinB by recombinant O. tauri Cdc25. We propose that there has been coevolution of the regulatory proteins involved in the control of M-phase entry in the metazoan, yeast and green lineages. |
| Databáze: | OpenAIRE |
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