Ultrastructural identification and distribution of the adhesion molecules ICAM-1 and LFA-1 in the vascular and extravascular compartments of the human palatine tonsil
| Autor: | K. A. Brown, B. von Gaudecker, M. E. Perry |
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| Rok vydání: | 1992 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Histology Endothelium Adolescent High endothelial venules Palatine Tonsil Plasma Cells Stratified squamous epithelium Biology Palatine tonsil Pathology and Forensic Medicine medicine Humans Child ICAM-1 Crypt Epithelium Cell adhesion molecule Antibodies Monoclonal Cell Biology Dendritic Cells Intercellular Adhesion Molecule-1 Immunohistochemistry Lymphocyte Function-Associated Antigen-1 medicine.anatomical_structure Child Preschool Basal lamina Endothelium Vascular Cell Adhesion Molecules |
| Zdroj: | Cell and tissue research. 268(2) |
| ISSN: | 0302-766X |
| Popis: | Immunohistological analysis of sections prepared from human palatine tonsils revealed marked differences in the distribution of the adhesion molecule, leucocyte function antigen-1 (LFA-1) and its counter receptor, intercellular adhesion molecule-1 (ICAM-1). Light microscopy showed that LFA-1 was restricted to the leucocytes, particularly the lymphocytes. In contrast, staining of ICAM-1 was predominantly confined to the vascular endothelium with the greatest expression seen on the morphologically distinct high endothelial venules in the parafollicular areas; these are the sites that appear to support lymphocyte migration. Electron microscopy revealed that ICAM-1 was present on the luminal and lateral surfaces of the high endothelium and absent from the abluminal surface supported by basal lamina. The ICAM-1 was also absent from those surfaces of the endothelium that were in close contact with intravascular lymphocytes. Other cells stained by the anti-ICM-1 antibody included dendritic cells, plasma cells and epithelial cells in the reticulated crypt epithelium and in the upper strata of the non-keratinised stratified squamous epithelium. The high expression of LFA-1 was most prominent on lymphocytes, low on antigen-presenting cells and activated lymphoid cells, and not detectable on plasma cells, epithelial and endothelial cells. We propose that LFA-1/ICAM-1 binding participates in mediating the transendothelial migration of lymphocytes across the high endothelial venules of palatine tonsil. |
| Databáze: | OpenAIRE |
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