Agarose gel filtration of concentrated fecal extracts containing virus-like particles associated with hepatitis A
| Autor: | C. R. Gravelle, Hollinger Fb, D. W. Bradley, C L Hornbeck |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Immunoradiometric assay
Pan troglodytes Hepatitis A Infection Size-exclusion chromatography Radioimmunoassay Biology Hepatitis A Chromatography Agarose Molecular biology Virus law.invention Absorbance Antigen-Antibody Reactions chemistry.chemical_compound Feces Microscopy Electron Infectious Diseases chemistry law Virology Agarose Animals Hepatovirus Electron microscope |
| Zdroj: | Intervirology. 6(4-5) |
| ISSN: | 0300-5526 |
| Popis: | Virus-like particles shown to be associated with hepatitis A infection were recently visualized by immune electron microscopy in human and chimpanzee acute-phase fecal extracts. Agarose gel filtration of concentrated chimpanzee fecal extracts containing those 27-nm diameter particles separated more than 99% of the high molecular weight fecal impurities into two major peaks as determined by absorbance at 280 and 260 nm. The hepatitis A-associated particles were found between these two peaks by both immune electron microscopy and a new immunoradiometric assay. |
| Databáze: | OpenAIRE |
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