Study of Lysozyme-Loaded Poly-L-Lactide (PLLA) Porous Microparticles in a Compressed CO2 Antisolvent Process
| Autor: | Xiao-Qian Su, Wu Wenguo, Ai-Zheng Chen, Yong-Qiang Kang, Shi-Bin Wang, Chen Zhao, Yuangang Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Materials science
PLLA lcsh:Technology chemistry.chemical_compound LSZ reduction formula Differential scanning calorimetry General Materials Science Thermal stability Fourier transform infrared spectroscopy Porosity lcsh:Microscopy lcsh:QC120-168.85 emulsion Lactide lcsh:QH201-278.5 lcsh:T PCA process porous microparticles chemistry Chemical engineering lcsh:TA1-2040 Emulsion pulmonary drug delivery lcsh:Descriptive and experimental mechanics Particle size lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Biomedical engineering |
| Zdroj: | Materials; Volume 6; Issue 8; Pages: 3571-3583 Materials, Vol 6, Iss 8, Pp 3571-3583 (2013) |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma6083571 |
| Popis: | Lysozyme (LSZ)-loaded poly-L-lactide (PLLA) porous microparticles (PMs) were successfully prepared by a compressed CO2 antisolvent process in combination with a water-in-oil emulsion process using LSZ as a drug model and ammonium bicarbonate as a porogen. The effects of different drug loads (5.0%, 7.5% and 10.0%) on the surface morphology, particle size, porosity, tapped density and drug release profile of the harvested PMs were investigated. The results show that an increase in the amount of LSZ added led to an increase in drug load (DL) but a decrease in encapsulation efficiency. The resulting LSZ-loaded PLLA PMs (LSZ-PLLA PMs) exhibited a porous and uneven morphology, with a density less than 0.1 g·cm−3, a geometric mean diameter of 16.9–18.8 μm, an aerodynamic diameter less than 2.8 μm, a fine particle fraction (FPF) of 59.2%–66.8%, and a porosity of 78.2%–86.3%. According to the results of differential scanning calorimetry, the addition of LSZ improved the thermal stability of PLLA. The Fourier transform infrared spectroscopy analysis and circular dichroism spectroscopy measurement reveal that no significant changes occurred in the molecular structures of LSZ during the fabrication process, which was further confirmed by the evaluation of enzyme activity of LSZ. It is demonstrated that the emulsion-combined precipitation with compressed antisolvent (PCA) process could be a promising technology to develop biomacromolecular drug-loaded inhalable carrier for pulmonary drug delivery. |
| Databáze: | OpenAIRE |
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