| Autor: |
Bohui Xiong, Yue Jiang, Yandi Wang, Xiao Han, Cong Zhang, Ruqing Zhong, Wei Ge, Baoquan Han, Zhaojia Ge, Gui'an Huang, Shen Yin, Wei Shen, Qingyuang Sun, Zhongyi Sun, Yong Zhao, Hongfu Zhang |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
AndrologyREFERENCES. 10(8) |
| ISSN: |
2047-2927 |
| Popis: |
Human sperm concentration and motility have dropped dramatically (50%) in the past few decades, and environmental factors are involved in this decline. Long non-coding RNAs (lncRNA) have been discovered to be involved in many cellular processes including spermatogenesis.This investigation aimed to explore the role of lncRNA8276 in murine spermatogenesis.The expression of lncRNA8276 was modified by knockdown or overexpression in mouse testes and spermatogonial stem cells (C18-4 cell line). Sperm quality was determined in the F0 and F1 generations of mice. Furthermore, the underlying mechanisms were studied through gene expression and/or protein expression of spermatogenesis-related genes and cell junction-related genes by different methods.In the current investigation, we discovered that sperm lncRNA8276 was decreased by NHOur study suggests that lncRNA8276 may be involved in cell-cell junction formation in the mouse testis to regulate spermatogenesis. It may be a target for the modification of spermatogenesis and male fertility, or male contraception. This investigation offers a potential therapeutic strategy for male infertility. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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