Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic β-cells
| Autor: | Charles H. Scudamore, David M. Hudson, Susan B. Curtis, Alison M.J. Buchan, Tanya A. M. Griffiths, Valerie C. Smith, Ann Y. K. Wong, Ross T. A. MacGillivray |
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| Rok vydání: | 2010 |
| Předmět: |
Hephaestin
Duodenum Physiology Enterocyte Gene Expression Myenteric Plexus Brunner Glands Biology Ferroxidase activity Antibodies Enteric Nervous System Antibody Specificity Ileum Insulin-Secreting Cells Physiology (medical) Pyloric Antrum medicine Humans Insulin Cation Transport Proteins Pancreas Antrum Neurons Gastrointestinal tract Hepatology Gastroenterology Ceruloplasmin Membrane Proteins Epithelial Cells Submucous Plexus Molecular biology Recombinant Proteins Gastrointestinal Tract Enterocytes Jejunum medicine.anatomical_structure Immunology biology.protein Enteric nervous system |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 298:G425-G432 |
| ISSN: | 1522-1547 0193-1857 |
| Popis: | Hephaestin (Hp) is a membrane protein with ferroxidase activity that converts Fe(II) to Fe(III) during the absorption of nutritional iron in the gut. Using anti-peptide antibodies to predicted immunogenic regions of rodent Hp, previous immunocytochemical studies in rat, mouse, and human gut tissues localized Hp to the basolateral membranes of the duodenal enterocytes where the Hp was predicted to aid in the transfer of Fe(III) to transferrin in the blood. We used a recombinant soluble form of human Hp to obtain a high-titer polyclonal antibody to Hp. This antibody was used to identify the intracellular location of Hp in human gut tissue. Our immunocytochemical studies confirmed the previous localization of Hp in human enterocytes. However, we also localized Hp to the entire length of the gastrointestinal tract, the antral portion of the stomach, and to the enteric nervous system (both the myenteric and submucous plexi). Hp was also localized to human pancreatic β-cells. In addition to its expression in the same cells as Hp, ferroportin was also localized to the ductal cells of the exocrine pancreas. The localization of the ferroxidase Hp to the neuronal plexi and the pancreatic β cells suggests a role for the enzymatic function of Hp in the protection of these specialized cell types from oxidative damage. |
| Databáze: | OpenAIRE |
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