Comparison of the production of eicosanoids by human and rat peritoneal macrophages and rat Kupffer cells
| Autor: | A. Brouwer, A.M.W.C. van den Broek, J. E. Vincent, J. H. P. Wilson, Freek J. Zijlstra, R. J. T. Ouwendijk |
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| Rok vydání: | 1988 |
| Předmět: |
Thromboxane
Leukotriene B4 Kupffer Cells Eicosatetraenoic acid Lipoxygenase Prostaglandin Arachidonic Acids Biology Biochemistry chemistry.chemical_compound Endocrinology Hydroxyeicosatetraenoic Acids Animals Ascitic Fluid Humans Peritoneal Lavage Peritoneal Cavity Chromatography High Pressure Liquid Arachidonic Acid Macrophages Rats Thromboxane B2 chemistry Prostaglandin-Endoperoxide Synthases biology.protein Fatty Acids Unsaturated Prostaglandins Arachidonic acid Female SRS-A Cyclooxygenase Prostaglandin D2 |
| Zdroj: | Prostaglandins. 35(3) |
| ISSN: | 0090-6980 |
| Popis: | Human and rat peritoneal macrophages and rat Kupffer cells were labelled with [1-14C] arachidonic acid and stimulated with the calcium ionophore A23187. The metabolites formed were separated by high pressure liquid chromatography (HPLC). Human peritoneal macrophages formed especially leukotriene B4, 5-hydroxy-6,8,11,14 eicosatetraenoic acid and small amounts of leukotriene C4 and thromboxane B2, 12-hydroxy-5,8,10 heptadecatrienoic acid and 6-keto-prostaglandin F1 alpha, whereas rat peritoneal macrophages mainly produced cyclooxygenase products and in particular thromboxane B2 and 12-hydroxy-5,8,10 heptadecatrienoic acid. Rat Kupffer cells synthesized mainly cyclooxygenase products such as prostaglandin F2 alpha, prostaglandin D2 and prostaglandin E2. These results indicate that the profile of eicosanoids production by macrophages is dependent both on the species and on the tissue from which the macrophage is derived. |
| Databáze: | OpenAIRE |
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