Serum inflammatory profiles in cystic fibrosis mice with and without Bordetella pseudohinzii infection
Autor: | Alexander Day, Rebecca J. Darrah, Paul Litman, Thomas J. Kelley |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
Cystic Fibrosis Bordetella Science Cystic Fibrosis Transmembrane Conductance Regulator Inflammation Systemic inflammation Cystic fibrosis Article Mice Immune system medicine Animals Bordetella Infections Multidisciplinary biology medicine.diagnostic_test business.industry medicine.disease Mucus Cystic fibrosis transmembrane conductance regulator Mice Inbred C57BL Disease Models Animal Chronic infection Bronchoalveolar lavage Mutation Immunology biology.protein Cytokines Medicine Female medicine.symptom Infection business |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Cystic fibrosis (CF) is an autosomal recessive disease caused by dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) protein, and is marked by an accumulation of mucus in affected airways resulting in persistent infection and chronic inflammation. Quantitative differences in inflammatory markers have been observed in CF patient serum, tracheal cells, and bronchoalveolar lavage fluid, in the absence of detectable infection, implying that absent CFTR function alone may result in dysregulated immune responses. To examine the relationship between absent CFTR and systemic inflammation, 22 analytes were measured in CF mice (F508del/F508del) sera using the MSD multiplex platform. Pro-inflammatory cytokines IL-2, TNF-α, IL-17α, IFN-γ, IL-1β, and MIP-3α are significantly elevated in infection-naïve CF mice (p p = 0.00003, p = 0.004). Additionally, six general markers of inflammation are significantly different from non-CF controls (p Bordetella pseudohinzii infections. There are no statistical differences in nearly all inflammatory markers when compared to their infection-naïve CF counterparts, except in the Th2-derived IL-4 and IL-5 which demonstrate significant decreases following exposure (p = 0.046, p = 0.045). Lastly, following acute infection, CF mice demonstrate elevations in nearly all inflammatory markers, but exhibit a shortened return to uninfected levels over time, and suppression of Th1-derived IL-2 and IL-5 (p = 0.043, p = 0.011). These results imply that CF mice have a persistent inflammatory profile often indistinguishable from chronic infection, and a dysregulated humoral response during and following active infection. |
Databáze: | OpenAIRE |
Externí odkaz: |