Serum albumin is essential for in vitro growth of activated human lymphocytes
| Autor: | H Spieker-Polet, H Polet |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Immunology Serum albumin Plasma protein binding In Vitro Techniques Biology Lymphocyte Activation Tuberculin chemistry.chemical_compound Concanavalin A Humans Immunology and Allergy Lymphocytes Cells Cultured Serum Albumin Albumin Articles Blood Proteins Blood proteins Culture Media Allogeneic Lymphocyte Biochemistry chemistry biology.protein Mitogens Hormone |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | The effect of human plasma, the plasma protein fractions of Cohn, and crystallized serum albumin on the in vitro growth of human lymphocytes activated by concanavalin A (Con A) or bacterial lipopolysaccharide was compared. It was found that fraction V or serum albumin (SA) is essential for growth of activated T and B lymphocytes. The other plasma proteins have no effect. The growth response of Con A-activated T lymphocytes to increasing concentrations of SA is similar to the response to increasing equivalent concentrations of plasma suggesting but not proving that SA is the only growth-stimulating factor in plasma when added to a protein-free culture medium. The growth-promoting effect of SA is not due to the fatty acids or hormones bound to SA but is attributed to the albumin molecule itself or to a factor tightly bound to it. SA can also effectively replace plasma to stimulate proliferation of lymphocytes activated by allogeneic lymphocytes or purified protein derivative of tuberculin. |
| Databáze: | OpenAIRE |
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