Hydration of single-stranded phosphodiester and phosphorothioate oligodeoxyribonucleotides
| Autor: | J. W. Powell, K. K. Reeves, E. Snyder, Richard H. Griffey, A. P. White, Henri Sasmor, Venkatraman Mohan, J. Farrell |
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| Rok vydání: | 1996 |
| Předmět: |
Molecular Sequence Data
DNA Single-Stranded Infrared spectroscopy Biology Adsorption Spectroscopy Fourier Transform Infrared Genetics Molecule Nucleotide Glycosides Furans chemistry.chemical_classification Base Sequence Water Thionucleotides Organophosphates Crystallography Models Chemical Oligodeoxyribonucleotides Biochemistry chemistry Phosphodiester bond Nucleic Acid Conformation Gravimetric analysis Absorption (chemistry) Hydrate Research Article |
| Zdroj: | Nucleic Acids Research. 24:3261-3266 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/24.16.3261 |
| Popis: | Infrared spectroscopy was used to identify hydration-sensitive structural differences between single- stranded phosphorothioate (PS) and phosphodiester (PO) oligodeoxyribonucleotides. Spectra were recorded in the mid-infrared region, 500-1800 cm-1, at relative humidities between 0 and 98%; the PS and PO spectra are substantially different. The hydration effects on spectral bands in these single-stranded oligodeoxyribonucleotides is markedly different from such behavior in double- and triple-stranded oligodeoxyribonucleotides. A strong absorption occurs at 656 cm-1 in the phosphorothioate sample which is completely absent from the PO spectra. Gravimetric measurements were carried out on one PS and one PO sample to monitor and confirm hydration. The calculated BET adsorption constants [Brunauer, S., Emmett, RH. and Teller, E. (1938) J. Am. Chem. Soc., 60, 309-319] are 1.2 and 1.4 water molecules per nucleotide in the first hydration layer of PS and PO respectively. While the gravimetric data indicate that the single-stranded oligodeoxyribonucleotides hydrate very similarly to duplex DNA, the mid-infrared conformational marker bands are strikingly different from those observed for duplex DNA. In particular, the Vas of the phosphate group (PO2) at 1222 cm-1 in the single-stranded PO spectra is independent of relative humidity. |
| Databáze: | OpenAIRE |
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